棚栽与瓶栽金线莲的广靶代谢组学比较  

Comparative analysis of widely targeted metabolomics between greenhouse-cultivated and bottle-acclimated Anoectochilus roxburghii

作　　者：邱道寿[1] 罗永坚 郑武林 李莉 QIU Daoshou;LUO Yongjian;ZHENG Wulin;LI Li(Crops Research Institute,Guangdong Academy of Agricultural Sciences/Key Laboratory of Crops Genetics&Improvement of Guangdong Province,Guangzhou,Guangdong 510640,China;School of Life Science and Technology,Central South University of Forestry and Technology,Changsha,Hunan 410004,China;Huaiji Caiyi Agricultural Technology Co.,Ltd.,Huaiji,Guangdong 526454,China;Longmen County Hujinyuan Ecological Agriculture Development Co.,Ltd.,Longmen,Guangdong 516820,China)

机构地区：[1]广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室,广东广州510640 [2]中南林业科技大学生命科学与技术学院,湖南长沙410004 [3]怀集彩毅农业科技有限责任公司,广东怀集526454 [4]龙门县斛金缘生态农业发展有限公司,广东龙门516820

出　　处：《亚热带农业研究》2024年第2期73-82,共10页Subtropical Agriculture Research

基　　金：广东省省级科技计划项目“药用及药食两用作物种质资源收集、鉴评与利用(2019B030316008)”;广东省科技计划项目“广东省农作物遗传改良重点实验室(2023B1212060038)”;广东省林业科技创新项目“林下南药种质资源收集保存与种植示范建设(2021KJCX013)”。

摘　　要：[目的]基于广泛靶向代谢组学技术,探索瓶栽和棚栽金线莲主要代谢产物的差异,为鉴别不同栽培方式的金线莲产品提供依据。[方法]以金线莲主栽品种‘红霞’为研究对象,采用UPLC-MS/MS分析技术对比了组培瓶苗驯化6月龄(瓶栽)与大棚栽培6月龄(棚栽)的金线莲全草干品之间的差异代谢物,并进行了标识代谢物筛选和差异代谢物通路富集分析。[结果](1)棚栽和瓶栽金线莲平均株高分别为11.37、9.90 cm,叶片数分别为8.75、5.75片,单株干质量分别为0.43、0.23 g,棚栽均极显著高于瓶栽;瓶栽和棚栽金线莲苷含量分别为138.75、144.75 g·kg^(-1),蛋白质含量分别为129.75、133.13 g·kg^(-1),棚栽略高于瓶栽,但两者差异不显著。(2)在两种干品中共检测到880种代谢物,从中筛选到差异代谢物89种,其中棚栽有55种上调表达、34种下调表达。含量较高的标识代谢物中,棚栽金线莲有22种,主要为黄酮醇、酚酸类和核苷酸及其衍生物等化合物;瓶栽有10种,主要为酚酸类、生物碱和游离脂肪酸等化合物。(3)通路富集分析结果显示,89种显著差异代谢物分布在48条代谢通路中,显著富集的有硫胺代谢,VB_(6)代谢,丙氨酸、天冬氨酸和谷氨酸代谢,生物素代谢,泛醌和其他萜类醌生物合成,黄酮和黄酮醇生物合成,嘌呤代谢,氨酰基tRNA生物合成,氰基氨基酸代谢,醚脂代谢,叶酸生物合成等途径。[结论]仅检测金线莲苷和蛋白质含量,无法区分瓶栽和棚栽金线莲产品。在差异代谢谱中,二者各有上下调表达,差异代谢物数量和含量都以棚栽金线莲具有显著优势,可为两种栽培品的鉴别提供依据。此外,在标识性代谢物中,金线莲的有效成分黄酮类、酚酸类等,也以棚栽具有显著优势,可为金线莲高质量栽培提供参考。[Purpose]Based on widely targeted metabolomics techniques,this study aimed to explore the differences in major metabolites between bottle-acclimated and greenhouse-cultivated Anoectochilus roxburghii.The study provided a theoretical basis for the identification of A.roxburghii products obtained using different cultivation methods.[Method]This study focused on the main cultivated variety of A.roxburghii,‘Hongxia’.UPLC-MS/MS analysis was used to compare and analyze the differential metabolites between the 6-month-old bottle-acclimated and the 6-month-old greenhouse-cultivated‘Hongxia’dried whole plant.Indicative and differential metabolite enrichment pathway analyses were also performed.[Result]After testing,the average plant height of A.roxburghii cultivated in the greenhouse and acclimated in the bottle was 11.37 cm and 9.90 cm,the average number of leaves was 8.75 and 5.75,while the average dry weight per plant was 0.43 g and 0.23 g,respectively.These results suggested that greenhouse cultivation was significantly more effective than bottle acclimatization.The kinsenoside content of bottle-acclimated and greenhouse-cultivated A.roxburghii was 138.75 g·kg^(-1)and 144.75 g·kg^(-1),respectively.The protein content was 129.75 g·kg^(-1)and 133.13 g·kg^(-1),respectively.Both were slightly higher in the greenhouse-cultivated products than in the bottle-acclimated products;however,the difference between the two was not significant.880 metabolites were detected in both the bottle-acclimated and greenhouse-cultivated dried samples,and 89 significantly different metabolites were identified.Among these,55 metabolites were upregulated during greenhouse cultivation and 34 metabolites were downregulated.Among the indicative metabolites with higher contents,there were 22 species in the greenhouse cultivation,including flavonoids,phenolic acids,nucleotides,and their derivatives.There were 10 types of compounds in bottle acclimation,including phenolic acids,alkaloids,and free fatty acids.Pathway enrichment analysis re

关 键 词：金线莲 大棚栽培 驯化瓶苗 代谢组学 标识代谢物 

分 类 号：S567.23[农业科学—中草药栽培] R282.2[农业科学—作物学]