紫苏磷脂酰乙醇胺结合蛋白基因家族成员PfFT3调控开花功能  

Functional Studies on the Regulation of Flowering by PfFT3,a Member of the Perilla PEBP Gene Family

作　　者：王其凤 冯小燕 李慧 侯福朋 郭曦 陆俊杏[1] 胡建 张涛[1] WANG Qi-Feng;FENG Xiao-Yan;LI Hui;HOU Fu-Peng;GUO Xi;LU Jun-Xing;HU Jian;ZHANG Tao(Chongqing Key Laboratory of Plant Environmental Adaptation Biology College of Life Sciences,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]植物环境适应生物学重庆市重点实验室,重庆师范大学生命科学学院,重庆401331

出　　处：《中国生物化学与分子生物学报》2024年第8期1173-1184,共12页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.31171588);重庆市自然科学基金创新发展联合基金项目(No.CSTB2024NSCQ-LZX0002);重庆市教委科学技术研究计划项目(No.KJZD-K202200508);人才引进项目博士启动基金(No.202311000481)资助。

摘　　要：紫苏(Perilla frutescens)含有丰富的生物活性物质和营养成分,是严格的短日照植物。目前,对紫苏的研究主要集中在产量和脂肪酸积累等农艺性状,而对紫苏开花进程及花器官形成的研究较少,其分子调控机制尚不明确。FLOWERING LOUC T(FT)是目前公认的感知光周期的关键基因,在花转变中发挥重要作用。PfFT3是FT-like亚家族基因,其在植物开花调控的功能尚待揭示。本研究首先通过亚细胞定位证明PfFT3定位于细胞核和细胞质。之后构建植物表达载体pCAMBIAI1303-PfFT3,并通过农杆菌介导的花序浸染法转化野生型Col-0和突变体fd-2、fd-3和ft-10拟南芥,以此分别获得稳定遗传的纯合的过表达和回补转基因株系。结果分析表明,PfFT3的过表达能够显著促进拟南芥开花并且能够挽救突变体fd-2、fd-3和ft-10的晚花表型,进一步研究表明,外源PfFT3基因的表达促进下游内源开花基因AtSOC1、AtAP1、AtFUL和AtLFY的表达。综上所述,本研究阐明了PfFT3在促进开花过程的积极作用,为深入研究PfPEBP基因的功能及紫苏短日照早花优良种质培育奠定基础。Perilla frutescens,a short-day plant,is rich in biologically active substances and nutrients.Current research on Perilla frutescens focuses on agronomic traits such as yield and fatty acid accumulation,with limited exploration of the flowering process and floral organ development.The molecular regulatory mechanisms underlying these aspects remain unclear.FLOWERING LOUC T(FT)is a florigen in Arabidopsis,plays critical roles in floral transition.PfFT 3 is unannotated by genome but annotated by transcriptomics data to the FT-like subfamily.Its function in controlling flowering is yet to be explored.Here subcellular localization analysis showed that PfFT3 is localized in the nucleus and cytoplasm.The plant over-expression vector pCAMBIAI1303-PfFT3 was constructed and transformed into wild-type(Col-0)and mutant fd-2,fd-3,and ft-10 plants by agrobacterium-mediated inflorescence infiltration as a means of obtaining genetically stable and pure overexpression and backfill transgenic lines in Arabidopsis,respectively.Analysis of the results showed that overexpression of PfFT 3 significantly promoted early flowering in Arabidopsis and rescued the late-flowering phenotype of the mutants fd-2,fd-3,and ft-10,and that expression of the exogenous PfFT 3 promoted the expression of the downstream endogenous flowering genes AtSOC1,AtAP1,AtFUL,and AtLFY.This study demonstrates the positive role of PfFT3 in promoting flowering,providing a foundation for further investigation of PfPEBP function and advancing the breeding of early-flowering Perilla frutescens cultivars.

关 键 词：紫苏 FLOWERING LOUC PfFT3 开花 

分 类 号：Q94[生物学—植物学]