新型水中总大肠菌群与大肠埃希氏菌检测方法的应用分析  

Application Analysis of a New Method for Determination of Total Coliform and Escherichia coli in Water

作　　者：曹新垲 张琦 张明明 尹宝国 张宝华 CAO Xinkai;ZHANG Qi;ZHANG Mingming;YIN Baoguo;ZHANG Baohua(Beijing Monitoring Station of National Urban Water Quality Monitoring Network,Beijing100101,China;Water Quality Monitoring Center,Beijing Waterworks Group Co.,Ltd.,Beijing100101,China)

机构地区：[1]国家城市供水水质监测网北京监测站,北京100101 [2]北京市自来水集团有限责任公司水质监测中心,北京100101

出　　处：《净水技术》2024年第8期83-88,共6页Water Purification Technology

摘　　要：为应对水质突发情况,减少检测时间,提高检测效率,文章对全自动微生物检测分析仪法的检测准确度、检测时间做了针对性的研究,并与国标中检测方法(酶底物法、滤膜法)检测实际样品的结果进行了对比分析。利用全自动微生物检测分析仪法分别检测高、低两种浓度的质控样品发现,所有检测结果均符合质控真值要求,检测结果可靠。利用梯度稀释并记录检测结果与时间发现,当样品浓度为0~1.0×10^(8)CFU/(100 mL)时,检测时间为2~18 h,样品浓度越高,检测时间越短。实际水样检测对比发现,全自动微生物检测分析仪法分别与国标中检测方法(滤膜法、酶底物法)检测结果配对t检验,总大肠菌群的配对t检验结果为P=0.122(>0.05)与P=0.351(>0.05),无显著性差异;大肠埃希氏菌的配对t检验结果为P=0.086(>0.05)与P=0.082(>0.05),无显著差异性。在保证数据准确性的前提下,利用全自动微生物检测分析仪法检测可以大幅缩短检测时间,适宜检测各类应急水质情况。In order to deal with water quality emergencies,the detection time is further reduced and the detection efficiency is improved.In this paper,the detection accuracy and detection time of automatic microbiological detection analyzer method were targeted researched,and the results of the raw water sample detected by the national standard detection methods(enzyme substrate method and filter membrane method)were compared and analyzed.The automatic microbiological detection analyzer method was used to detect the high and low concentrations of quality control samples,and it was found that all the test results met quality control truth value requirements,the results of this method were reliable.Using serial dilutions and recording of test results and time,it was found that the detection time was from 2 to 18 hours with the sample concentration from 0 to 1.0×10^(8)CFU/(100 mL),the higher the sample concentration,the shorter the detection time.The comparison of raw water samples showed that,the automatic microbiological detection analyzer method paired with the detection results of the national standard detection method(filter membrane method and enzyme substrate method),and the paired t-test results of total coliform were P=0.122(>0.05)and P=0.351(>0.05),with no significant difference;meanwhile the paired t-test results of Escherichia coli were P=0.086(>0.05)and P=0.082(>0.05),with no significant difference.On the premise of ensuring data accuracy,the detection time can be greatly shortened by using the automatic microbiological detection analyzer method,which is suitable for detecting all kinds of emergency water quality conditions.

关 键 词：总大肠菌群 大肠埃希氏菌 全自动微生物检测分析仪 滤膜法 酶底物法 

分 类 号：TU991[建筑科学—市政工程]