高州油茶糖积累及其代谢相关酶活性  

Sugar Accumulation and the Relevant Enzymes Activities of Camellia drupifera

作　　者：刘祯 陈锐帆 申春晖 奚如春 邓小梅 LIU Zhen;CHEN Rui-fan;SHEN Chun-hui;XI Ru-chun;DENG Xiao-mei(College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,Guangdong,China;Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm,Guangzhou 510642,Guangdong,China)

机构地区：[1]华南农业大学林学与风景园林学院,广东广州510642 [2]广东省森林植物种质创新与利用重点实验室,广东广州510642

出　　处：《林业科学研究》2024年第4期41-51,共11页Forest Research

基　　金：广东省重点领域研发计划项目(2020B020215003);广东省林业科技创新项目(2018KJCX008)。

摘　　要：[目的]研究高州油茶生长发育过程中各源库器官糖积累特征及其与糖代谢相关酶活性的关系,为进一步完善华南地区特色油茶品种糖积累理论提供依据。[方法]以高州油茶为研究对象,利用13C同位素脉冲标记法测定不同发育时期各源库器官的13C含量,同时测定对应时期各器官非结构性碳水化合物(NSC)含量、糖组分特征及相关酶活性的变化。[结果]1)13C标记的光合产物在运输趋于稳定后,在发育前期主要积累在叶片中,后期种仁逐渐成为了同化物分配积累的中心。2)各器官可溶性糖含量基本表现为果皮>种仁>叶片,淀粉含量表现为叶片>种仁>果皮。3)SPS和SS-Ⅱ与叶片、果皮和种仁的可溶性糖含量都呈正相关关系;B-AI主要在发育后期起着作用,种仁中B-AI活性分别在8月和10月存在峰值;在叶片中,NI为主要的糖转化酶,活性在50~60 U·g^(−1)之间;种仁中,S-AI与NI的活性大致相同,S-AI主要在发育前期起作用,而NI在发育后期起作用。[结论]高州油茶发育前期光合产物主要积累在源器官叶片之中,随着果实生长发育种仁逐渐成为了同化物分配的中心;在糖积累情况上,种仁在发育前期主要积累果糖和葡萄糖,发育后期则主要积累蔗糖。在发育后期运输到果皮的蔗糖会分解为果糖和葡萄糖,果皮依旧会进行增厚和延伸,继续完成木质化,此时果皮与种仁会对养分进行激烈竞争,最终呈现出果大皮厚的现象。SPS和SS-Ⅱ功能互补,同时影响着高州油茶糖分的积累,且SS-Ⅱ是糖分积累的关键酶;可能是引起各源库器官糖含量差异的主要原因,高州油茶糖积累受糖代谢相关酶的综合调控,NI为叶片的主要转化酶,S-AI与NI分别在种仁发育前后期发挥作用,果皮中蔗糖转化主要依靠S-AI;B-AI主要在发育后期起作用,但活性相对较低,转化能力有限。[Objective]To study the characteristics of sugar accumulation in various source-sink organs during the growth and development of Camellia drupifera and its relationship with the enzymatic activities related to sugar metabolism for providing a basis for further improving the theory of sugar accumulation in characteristic oil tea camellia varieties in the South China region.[Method]The 13C isotope pulse labeling method was employed to mesure the 13C content in various source and sink organs at different developmental stages of C.drupifera.Concurrently,the non-structural carbohydrate(NSC)content,sugar component characteristics,and related enzyme activities in the respective organs during the corresponding periods.[Result]1)After the stabilization of the transport of 13C-labeled photosynthates,during the early developmental stages,these products were primarily accumulated in the eaves,while in the later stages,the seed kernels gradually became the central location for assimilate distribution and accumulation.2)The soluble sugar content in various organs was mainly observed in the order of peels>seeds>leaves,while starch content showed the order of leaves>seeds>peels.3)The activities of SPS and SS-II were positively correlated with the soluble sugar content in leaves,peels,and seeds.B-AI primarily played a role during the later stages of development,with peak activities in seed kernels occurring in August and October.In leaves,NI served as the primary sugar-converting enzyme,with activity ranging between 50 and 60 U·g^(−1).In seed kernels,the activities of S-AI and NI were roughly similar,with S-AI primarily acting during the early developmental stages,while NI was more active during the later stages of development.[Conclusion]In C.drupifera,during the early stages of development,photosynthates primarily accumulate leaves of the source organ,.As fruit grows,the seed kernels gradually becomes the central location for assimilate distribution.Regarding sugar accumulation,during the early developmental stages,seed kern

关 键 词：糖含量 酶活性 13C脉冲标记 高州油茶 

分 类 号：S794.4[农业科学—林木遗传育种]