不同外源物质对蒙古栎插穗生根及基部生理生化的影响  

Effects of Different Exogenous Substances on Rooting,Basal Physiology and Biochemistry of Quercus mongolica Cuttings

作　　者：钟明霞 艾万峰 袁欣 黄一鹤 魏俊[1] 王玉涛[1] 陆秀君[1] ZHONG Ming-xia;AI Wan-feng;YUAN Xin;HUANG Yi-he;WEI Jun;WANG Yu-tao;LU Xiu-jun(College of Forestry,Shenyang Agricultural University,Shenyang 110866,Liaoning,China)

机构地区：[1]沈阳农业大学林学院,辽宁沈阳100866

出　　处：《林业科学研究》2024年第4期108-116,共9页Forest Research

基　　金：辽宁省教育厅基本科研项目:耐旱乡土树种干旱胁迫响应的分子机制(No.Z20240296)。

摘　　要：[目的]扦插是苗木良种繁育的一种重要途径,适宜外源物质的应用能促进插穗生根。蒙古栎扦插生根困难,探究不同浓度外源物质及其组合应用对蒙古栎嫩枝扦插生根的影响,为蒙古栎规模化扦插育苗提供技术支持。[方法]采用速蘸法或浸泡法,使用不同浓度吲哚丁酸钾(IBA-K)、生根粉(ABT1)、K599发根农杆菌、丙环唑(PCZ)及其组合应用处理插穗,测定插穗生根率、愈伤率、生根数、根长、营养物质含量、酶活性和内源激素含量变化等。[结果]不同外源物质单独处理时,K599发根农杆菌(OD600=0.8)浸泡30 min后插穗生根率最高,可达24.71%。50μmol·L^(−1)PCZ浸泡2 h后插穗根部愈伤率最高,达43.62%。其它外源物质单独处理时,插穗生根效果差。不同外源物质组合应用时,50μmol·L^(−1)PCZ浸泡2 h结合K599发根农杆菌(OD600=0.8)浸泡30 min可显著提高蒙古栎插穗生根,生根率可达36.37%,愈伤率达51.14%,平均生根数及根长分别为2.25和5.72 cm。此外,研究结果表明,PCZ能够加快插穗生根过程中可溶性糖和蛋白消耗,促进过氧化物酶(POD)和多酚氧化酶(PPO)活性,提高不定根诱导期IAA含量,增加IAA/ABA和IAA/ZR比值。[结论]PCZ结合发根农杆菌K599组合应用能够显著提高蒙古栎嫩枝扦插生根率。营养物质(可溶性糖、蛋白)消耗慢、抗氧化酶(PPO、POD)活性弱、IAA含量低可能是蒙古栎扦插生根难的主要原因,而PCZ对此具有重要抑制作用,可在蒙古栎等扦插难生根物种中发挥重要作用。[Objective]Cutting propagation is an important way to propagate excellent seedlings,and the application of suitable exogenous substances can promote rooting of cuttings.The rooting of Quercus mongolica cuttings is difficult.Exploring the effects of different concentrations of exogenous substances and their combine applications on the rooting of Quercus mongolica softwood cuttings can provide technical support for large-scale propagation by cutting propagation.[Method]Using rapid dipping or soaking methods,different concentrations of indole butyrate potassium(IBA-K),rooting powder(ABT1),K599 Agrobacterium rhizogenes,propiconazole(PCZ),and their combination were used to treat cuttings.The rooting rate,callus rate,rooting number,root length,nutrient content,enzyme activity,and changes in endogenous hormone content of cuttings were measured.[Result]When different exogenous substances were used separately,the rooting rate of K599 Agrobacterium rhizogenes(OD600=0.8)was the highest after soaking for 30 minutes,reaching 24.71%.After soaking in 50μM PCZ for 2 hours,the root callus rate of cuttings was the highest,reaching 43.62%.For other exogenous substances alone,the rooting effect of cuttings was poor.When different combinations of exogenous substances were used,50μmol·L^(−1) PCZ soaking for 2 hours combined with K599 Agrobacterium rhizogenes(OD600=0.8)soaking for 30 minutes significantly improved the rooting rate of Quercus mongolica cuttings,reaching 36.37%for rooting and 51.14%for callus.The average number of roots and root length were 2.25 and 5.72 cm,respectively.In addition,the results indicated that the use of PCZ can accelerate the consumption of soluble sugars and proteins during the rooting process of cuttings,promote the activities of peroxidase(POD)and polyphenol oxidase(PPO),increase the content of IAA during the induction period of adventitious roots,and increase the IAA/ABA and IAA/ZR ratios.[Conclusion]The combined application of PCZ and Agrobacterium rhizogenes K599 can significantly improve the r

关 键 词：扦插 发根农杆菌 丙环唑 生根机理 

分 类 号：S723.1[农业科学—林木遗传育种]