银白杨对干旱胁迫的生理响应研究  

The Physiological Response of Populus alba to Drought Stress

作　　者：徐慧 薛媛 XU Hui;XUE Yuan(State Key Laboratory of Tree Genetics and Breeding,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]林木遗传育种全国重点实验室中国林业科学研究院,北京100091

出　　处：《林业科学研究》2024年第4期166-173,共8页Forest Research

基　　金：国家自然基金青年科学基金项目(32001336);“十四五”国家重点研发计划课题(2022YFD2200102)。

摘　　要：[目的]以银白杨为研究对象,探究干旱胁迫对其生长发育的影响,为解析多年生林木响应干旱胁迫的分子机制奠定基础。[方法]对生长在土里5周左右的银白杨幼苗进行干旱胁迫处理,通过观察其生长状态,测定与干旱胁迫相关生理指标以及检测相关基因表达情况,探究银白杨对干旱胁迫的耐受能力。[结果]对银白杨植株进行干旱处理8 d后,其叶片含水量明显下降、气孔开度变小;ROS含量明显上升,体内ABA积累、而与生长相关的IAA含量下降;与干旱胁迫相关的基因表达上调。[结论]通过多角度探究银白杨对干旱胁迫的耐受性,银白杨幼苗在干旱处理8 d后其生长受到显著抑制,建议及时浇水。[Objective]In this study,Populus alba was used to detect the effect of drought stress on growth and development,which provided reference for the molecular mechanism of the response to drought stress in woody.[Method]The seedlings of Populus alba about five weeks were treated with drought stress,the growth state and drought stress-related physiological indicators and genes were record and detected to reveal the resistance of Populus alba to drought stress.[Result]The results showed that the water content of leaves decreased significantly and stomatal aperture became smaller after 8 days of drought treatment;meanwhile ROS increased significantly,ABA accumulated in the body,and the growth-related hormone IAA content decreased,drought stress-related genes were upregulated.[Conclusion]By detecting the tolerance of Populus alba to drought stress from multiple perspective,the growth of their seedlings is significantly inhibited after drought treatment for 8 days,which indicates that watering timely is necessary.

关 键 词：银白杨 干旱胁迫 生理指标 

分 类 号：S722.3[农业科学—林木遗传育种]