掩味型紫草红花乳膏剂抗炎抗真菌的作用研究  

Study on the Anti-Inflammatory and Antifungal Effects of Taste-Masked Lithospermum Safflower Emulsion

作　　者：袁文博 钟宏耀 成欣怡 魏坤 姚荧 YUAN Wenbo;ZHONG Hongyao;CHENG Xinyi;WEI Kun;YAO Ying(Affiliated Obstetrics and Gynecology Hospital of Jiangnan University,Wuxi 214002,China;Wuxi School of Medicine,Jiangnan University,Wuxi 214122,China;School of Pharmacy,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学附属妇产医院,江苏无锡214002 [2]江南大学无锡医学院,江苏无锡214122 [3]江南大学药学院,江苏无锡214122

出　　处：《南京中医药大学学报》2024年第8期812-822,共11页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：江苏省药学会靳培英基金项目(J2021003)。

摘　　要：目的探究掩味型紫草红花乳膏剂在体内和体外抗炎抗真菌的作用机制。方法体外采用MTT、qPCR和ELISA法测定其抗炎作用;通过肉汤稀释实验、杀菌动力学、芽管抑制、XTT还原试验等方法来考察掩味型紫草红花乳膏剂的抗真菌作用;体内实验研究通过建立小鼠白色念珠菌皮肤病模型,考察其疗效和机制。结果掩味型紫草红花乳膏剂中的紫草对RAW264.7细胞增殖有明显抑制作用,红花呈剂量依赖性地抑制LPS诱导的IL-6的生成。紫草显著抑制白色念珠菌活性,其杀菌模式具有时间和浓度依赖性;紫草能显著减少白色念珠菌芽管的形成,破坏其生物被膜;红花对白色念珠菌没有直接杀灭作用,不能抑制其生物被膜形成,但其显著减少白色念珠菌的菌丝生长,并提高其ROS水平。结论掩味型紫草红花乳膏剂中紫草和红花配伍,可以协同起效,具有减轻炎症损伤,治疗皮肤白色念珠菌感染的功效。OBJECTIVE To explore the anti-inflammatory and antifungal action mechanism of taste-masked Lithospermum Safflower emulsion in vivo and in vitro.METHODS In vitro,the anti-inflammatory effect was detected by MTT assay,qPCR and ELISA.The anti-fungal effect of the product was investigated by broth dilution experiment,bactericidal kinetics,germ tube inhibition and XTT reduction test.In vivo,the effect was evaluated and the mechanism was investigated on the skin disease model of Candida albicans in mice.RESULTS Lithospermum in taste-masked Lithospermum Safflower emulsion had a significant inhibitory effect on the proliferation of RAW264.7 cells,and Safflower inhibited the production of IL-6 induced by LPS in a dose-dependent manner.Lithospermum significantly inhibited the activity of Candida albicans,and its bactericidal mode is time-and concentration-dependent;Lithospermum significantly reduced the formation of Candida albicans germ tubes and destroyed its biofilm;Safflower had no direct killing effect on Candida albicans,was not able to inhibit its biofilm formation,but significantly reduced the hyphal growth of Candida albicans and increased its ROS level.CONCLUSION The combination of Lithospermum and Safflower in the taste-masked Lithospermum Safflower emulsion can work synergistically to reduce inflammatory damage and treat Candida albicans infection of the skin.

关 键 词：掩味型紫草红花乳膏剂 抗真菌 抗炎 白色念珠菌 

分 类 号：R285.5[医药卫生—中药学]