基于超高效液相色谱串联质谱法和网络药理学探究雨生红球藻提取物延缓皮肤衰老机制  

Study on mechanism of Haematococcus pluvialis extract in skin age-delayingbased on UPLC-MS/MS and network pharmacology

作　　者：唐荣 孙锦月 李钧翔 何聪芬[1] TANG Rong;SUN Jinyue;LI Junxiang;HE Congfen(College of Chemistry and Materials Science,Beijing Technology and Business University,Beijing 100048,China;AGECODE R&D CENTER Yangtze Delta Region Institute of Tsinghua University,Jiaxing 314006,China;Harvest Biotech Co.Ltd.,Jiaxing 314006,China)

机构地区：[1]北京工商大学化学与材料工程学院,北京100048 [2]浙江清华长三角研究院衰老科学创新研发中心,浙江嘉兴314006 [3]禾美生物科技有限公司,浙江嘉兴314006

出　　处：《食品与发酵工业》2024年第16期332-340,共9页Food and Fermentation Industries

摘　　要：该研究旨在通过超高效液相色谱串联质谱法(ultra performance liquid chromatography-mass spectrometry, UPLC-MS/MS)鉴定雨生红球藻提取物(Haematococcus pluvialis extract, HPE)的活性成分,用网络药理学预测HPE可能发挥延缓皮肤衰老作用的机制,并进行实验验证。采用中药系统药理学(traditional chinese medicine systems pharmacology, TCMSP)、SwissTargetPrediction、细胞衰老基因数据库CellAge等数据库收集HPE与衰老的交集靶点,基于蛋白互作网络筛选核心靶点与关键成分,并对核心靶点进行基因本体(gene ontology, GO)富集和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes, KEGG)通路富集分析。结果表明,HPE共有16种活性成分,对应196个靶点,蛋白互作网络筛选到30个核心靶点,对应14个关键成分。GO富集及KEGG通路富集分析表明,HPE可能通过调控丝裂原活化蛋白激酶信号通路、调节瞬时受体电位离子通道的炎症介质、核因子κB(nuclear factor kappa-B,NF-κB)信号通路等实现延缓皮肤衰老的作用。通过分子对接对关键成分与核心靶点的结合能力进行预测分析,对接分数显示关建成分和核心靶点有良好结合性。体外细胞实验证明,HPE可显著抑制小鼠单核巨噬细胞中炎症因子表达(P<0.05),及显著提高人包皮成纤维细胞经紫外光照射后Ⅰ型胶原蛋白水平(P<0.01)。HPE可能通过对于炎症性通路及炎症因子的调节延缓皮肤衰老,具有多成分-多靶点-多途径的特点。This study aimed to identify the active ingredient of Haematococcus pluvialis extract(HPE)by UPLC-MS/MS,to predict the mechanism of HPE in delaying skin aging by network pharmacology and to verify the mechanism experimentally.Based on TCMSP,SwissTargetPrediction,CellAge,and other databases that collect the intersection targets of HPE and aging,the protein-protein interaction networks were used to screen the core targets and key components,and gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed on the core targets.Results showed that there were 16 active ingredients of HPE,corresponding to 196 targets,and the protein-protein interaction networks screened 30 core targets,corresponding to 14 key ingredients.GO enrichment analysis and KEGG pathway enrichment analysis revealed the regulatory activity of HPE to inflammation through mitogen-activated protein kinase signaling,transient receptor potential ion channel mediator regulation,nuclear factor kappa-B signaling,etc.Molecular docking was used to predict the binding ability of key components to the core targets,and the docking scores showed good binding ability between the key components and the core targets.Furthermore,in vitro experiments showed that HPE could significantly inhibit the expression of inflammatory factors in mouse mononuclear macrophage cells and increase the collagenⅠlevel of human foreskin fibroblast cells upon ultraviolet A irradiation.HPE may delay skin aging through the regulation of inflammatory pathways and inflammatory factors and have multi-component-multi-target-multi-pathway characteristics.

关 键 词：雨生红球藻提取物 UPLC-MS/MS 网络药理学 皮肤衰老 

分 类 号：R285[医药卫生—中药学] O657.63[医药卫生—中医学]