山楂叶总黄酮通过miR-21a-5p对血管紧张素Ⅱ诱导的心肌细胞肥大的影响  

Effects of hawthorn leaves flavonoids on angiotensin Ⅱ-induced cardiac hypertrophy by regulating miR-21a-5p

作　　者：田林燕[1] 朱冉[1] 代聚平[3] 王佳佳 张斌[2] TIAN Lin-yan;ZHU Ran;DAI Ju-ping;WANG Jia-ja;ZHANG Bin(Department of Internal Medicine,Nanyang 473000,Henan Province,China;Clinical Practice Room,Nanyang Medical College,Nanyang 473000,Henan Province,China;Department of Cardiovascular Medicine,The First Affiliated Hospital of Nanyang Medical College,Nanyang 473000,Henan Province,China;Department of Pathology,The First Affiliated Hospital of Nanyang Medical College,Nanyang 473000,Henan Province,China)

机构地区：[1]南阳医学高等专科学校内科教研室,河南南阳473000 [2]南阳医学高等专科学校临床实训室,河南南阳473000 [3]南阳医学高等专科学校第一附属医院心血管内科,河南南阳473000 [4]南阳医学高等专科学校第一附属医院病理科,河南南阳473000

出　　处：《中国临床药理学杂志》2024年第15期2182-2186,共5页The Chinese Journal of Clinical Pharmacology

基　　金：河南省医学科技攻关基金资助项目(LHGJ20190505)。

摘　　要：目的探讨山楂叶总黄酮对血管紧张素Ⅱ(AngⅡ)诱导的心肌肥大的影响,及其可能作用机制。方法将H9c2细胞分为对照组(正常培养),模型组(100 nmol·L^(-1)AngⅡ处理24 h),低、中、高剂量实验组(先给予100 nmol·L^(-1)AngⅡ处理24 h后,再分别给予25、50、100 mg·L^(-1)的山楂叶总黄酮处理24 h),anti-miR-NC、anti-miR-21a-5p组(分别转染anti-miR-NC、anti-miR-21a-5p后,给予100 nmol·L^(-1)AngⅡ处理24 h),miR-NC+高剂量组和miR-21a-5p+高剂量组(分别转染miR-NC、miR-21a-5p mimics,再给予100 nmol·L^(-1)AngⅡ+100 mg·L^(-1)山楂叶总黄酮各处理24 h)。用CCK-8法和流式细胞术检测细胞活力和凋亡情况,用实时荧光定量聚合酶链反应法检测miR-21a-5p的表达水平,用蛋白质印迹法检测环氧化酶-2(COX2)/前列腺素E2(PGE2)的表达水平。结果对照组、模型组和高剂量实验组的细胞活力分别为1.03±0.09、0.51±0.05和0.93±0.08,凋亡率分别为(7.69±0.61)%、(23.04±1.82)%和(9.43±0.71)%,miR-21a-5p表达水平分别为1.00±0.09、2.43±0.18和1.09±0.08,COX2蛋白相对表达水平分别为0.42±0.03、0.85±0.08和0.40±0.04,PGE2蛋白相对表达水平分别为0.34±0.03、0.75±0.07和0.35±0.03,模型组的上述指标与高剂量实验组和对照组相比,在统计学上差异均有统计学意义(均P<0.05)。anti-miR-NC组、anti-miR-21a-5p组、miR-NC+高剂量组和miR-21a-5p+高剂量组的细胞活力分别为0.52±0.04、1.12±0.08、0.94±0.09和0.57±0.04,凋亡率分别为(23.04±1.82)%、(9.86±0.73)%、(9.47±0.64)%和(24.96±1.94)%,miR-21a-5p表达水平分别为1.00±0.10、0.43±0.04、1.00±0.09和2.12±0.18,COX2蛋白相对表达水平分别为0.86±0.05、0.39±0.04、0.41±0.03、0.78±0.07,PGE2蛋白相对表达水平分别为0.74±0.06、0.38±0.07、0.36±0.02、0.71±0.05,anti-miR-21a-5p组的上述指标与anti-miR-NC组比较,miR-21a-5p+高剂量组的上述指标与miR-NC+高剂量组比较,在统计学上差异均有统计学意义(均P<0.05)�Objective To explore the effect and mechanism of hawthorn leaves flavonoids(HLF)on angiotensin Ⅱ(AngⅡ)-induced myocardial hypertrophy.Methods The H9c2 cells were divided into control group(normal culture),model group(100 nmol·L^(-1)AngⅡ for 24 h),experimental-L,-M,-H groups(received 100 nmol·L^(-1)AngⅡ for 24 h,then treated with 25,50 and 100 mg·L^(-1)HLF for 24 h,respectively),anti-miR-NC and anti-miR-21a-5p groups(transfected withanti-miR-NCandanti-miR-21a-5p,then treated with 100 nmol·L^(-1)AngⅡ for 24 h),miR-NC+high-dose and miR-21a-5p+high-dose group(transfected withmiR-NCand miR-21a-5p mimics,then treated with 100 nmol·L^(-1)AngⅡ for 24 h+100 mg·L^(-1)HLF for 24 h).The cell viability was detected by cell counting kit-8.The cell apoptosis was measured by flow cytometry.The expression levels of miR-21a-5pwas assessed by quantitative real-time polymerase chain reaction.The expression levels of cyclooxygenase-2(COX2)and prostaglandin E2(PGE2)was measured by Western blot.Results The cell viabilities of control,model group experimental-H groups were 1.03±0.09,0.51±0.05 and 0.93±0.08;cell apoptosis rates were(7.69±0.61)%,(23.04±1.82)% and(9.43±0.71)%;the expression levels of miR-21a-5pwere 1.00±0.09,2.43±0.18 and 1.09±0.08;the relative expression levels of COX2 protein were0.42±0.03,0.85±0.08 and 0.40±0.04;the relative expression levels of PGE2 protein were 0.34±0.03,0.75±0.07 and 0.35±0.03;the differences of above indexes were statistically significant between the model group and the control and experimental-H groups(allP<0.05).The cell viabilities of anti-miR-NC,anti-miR-21a-5p,miR-NC+high dose and miR-21a-5p+high dose groups were 0.52±0.04,1.12±0.08,0.94±0.09 and 0.57±0.04;the cell apoptosis rates were(23.04±1.82)%,(9.86±0.73)%,(9.47±0.64)% and(24.96±1.94)%;the expression levels of miR-21a-5p were 1.00±0.10,0.43±0.04,1.00±0.09 and 2.12±0.18;the relative expression levels of COX2 protein were 0.86±0.05,0.39±0.04,0.41±0.03 and 0.78±0.07;the relative expression leve

关 键 词：山楂叶总黄酮 心肌肥大 血管紧张素Ⅱ 细胞增殖 凋亡 

分 类 号：R28[医药卫生—中药学]