炙红芪对脾气虚大鼠肠黏膜屏障保护的研究  

Study of honey-processed Hedysari Radix on the protectior of intestinal mucosal barrier in rats with spleen

作　　者：王毛毛 宋沁洁 王哲 马定财 张育贵 刘婷 张转红 高飞云 王彦钧 李越峰 WANG Mao-mao;SONG Qin-jie;WANG Zhe;MA Ding-cai;ZHANG Yu-gui;LIU Ting;ZHANG Zhuan-hong;GAO Fei-yun;WANG Yan-jun;LI Yue-feng(School of Pharmacy,Gansu University of Traditional Chinese Medicine,Lanzhou 7730000,Gansu Province,China;Gansu Provincial Key Laboratory of Quality and Standards of Traditional Chinese Medicine,Lanzhou 730000,Gansu Province,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]甘肃省中药质量与标准研究重点实验室,甘肃兰州730000

出　　处：《中国临床药理学杂志》2024年第15期2231-2235,共5页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81960713,82160750);甘肃省教育厅产业支撑计划基金资助项目(2021CYZC-21);甘肃省科技计划(创新基地与人才计划)基础研究创新群体基金资助项目(21JR7RA569)。

摘　　要：目的 探究炙红芪调节脾气虚大鼠肠黏膜损伤的保护作用机制。方法 用苦寒泻下、劳倦过度及饥饱失常三因素复合造模法构建脾气虚模型大鼠。待造模成功后,随机分为模型组、炙红芪组和益生菌组,每组15只;另取15只正常大鼠作为空白组。炙红芪组以12.6 g·kg^(-1)的剂量灌胃给予炙红芪水煎液,益生菌组以0.625 g·kg^(-1)的剂量灌胃给予双歧杆菌乳杆菌三联活菌片混悬液,空白组和模型组均灌胃给予等剂量蒸馏水。4组大鼠每天给药1次,持续干预15 d。用酶联免疫吸附试验法检测血清中二胺氧化酶(DAO)、D-乳酸(D-LA)、分泌型免疫球蛋白A(SIgA)因子的水平,用蛋白质印迹法检测结肠组织中丝氨酸/苏氨酸蛋白激酶(AMPK)、闭合小带蛋白1(ZO-1)、闭锁蛋白(occludin)的表达水平。结果 空白组、模型组、炙红芪组和益生菌组大鼠血清中DAO含量分别为(138.93±9.78)、(187.95±12.90)、(147.21±6.92)和(166.47±3.37)pg·mL^(-1),D-LA含量分别为(892.23±49.17)、(1 099.84±137.64)、(956.56±86.04)和(989.61±51.75)μg·L^(-1),结肠组织中SIgA含量分别为(14.04±1.42)、(11.47±2.39)、(11.84±1.49)和(12.93±1.65)μg·mL^(-1),结肠组织中ZO-1蛋白相对表达水平分别为1.18±0.11、0.42±0.04、0.77±0.05和0.95±0.07,occludin蛋白相对表达水平分别为1.35±0.31、0.61±0.17、1.19±0.19和0.88±0.13,AMPK蛋白相对表达水平分别为0.91±0.02、0.35±0.09、0.74±0.08和0.59±0.11。炙红芪组与模型组相比,大鼠血清中DAO、D-LA含量,结肠组织中SIgA含量及ZO-1、occludin、AMPK蛋白相对表达水平在统计学上差异均有统计学意义(P<0.01,P<0.05)。结论 炙红芪可通过调节脾气虚大鼠机体中相关炎症细胞因子、肠黏膜上层细胞酶、紧密连接蛋白等的表达,增强对脾气虚大鼠肠道黏膜的保护作用。Objective To explore the protective mechanism of honey-processed Hedysari Radix in regulating intestinal mucosal injury in rats with spleen qi deficiency.Methods The three-factor composite modeling method of bitter cold diarrhea,overwork and hunger and satiety disorder was used to construct a spleen qi deficiency model rats.After the model was successfully made,they were randomly divided into model group,honey-processed Hedysari Radix group and probiotic group,with 15 animals in each group.Another 15 normal rats were taken as the blank group.The honey-processed Hedysari Radix group was given 12.6 g·kg^(-1)water decoction of honey-processed Hedysari Radix by gavage,the probiotics group was given Bifidobacterium Lactobacillus triple viable tablets suspension at a dose of 0.625 g·kg^(-1),and the blank group and the model group were given the same dose of distilled water.The rats in the four groups were administered once a day for 15 days.Enzyme-linked immunosorbent assay was used to detect diamine oxidase(DAO)in serum,D-lactic acid(D-LA),secretory immunoglobulin A factor,and Western blotting was used to detect the expression levels of AMP-activated protein kinase(AMPK),zonula occludens-1(ZO-1)and occludin in colon tissues.Results The serum levels of DAO inthe blank group,model group,honey-processed Hedysari Radix group and probiotic group were(138.93±9.78),(187.95±12.90),(147.21±6.92)and(166.47±3.37)pg·mL^(-1);the contents of D-LA were(892.23±49.17),(1099.84±137.64),(956.56±86.04)and(989.61±51.75)μg·L^(-1);the contents of SIgA in colon tissues were(14.04±1.42),(11.47±2.39),(11.84±1.49)and(12.93±1.65)μg·mL^(-1);therelative expression levels of ZO-1 protein in colon tissues were 1.18±0.11,0.42±0.04,0.77±0.05 and 0.95±0.07;the relative expression levels of occludin protein were 1.35±0.31,0.61±0.17,1.19±0.19 and 0.88±0.13;the relative expression levels of AMPK protein were 0.91±0.02,0.35±0.09,0.74±0.08 and 0.59±0.11.Compared with the model group,there were significant differences in the se

关 键 词：炙红芪 脾气虚证 肠黏膜屏障 保护作用 

分 类 号：R28[医药卫生—中药学]