三仁汤对幽门螺杆菌相关性胃炎脾胃湿热证大鼠消化吸收功能及Akt/NF-κB通路的影响  

Effect of Sanren Decoction on Digestion and Absorption Function and Akt/NF-κB Pathway in Rats with Helicobacter Pylori-associated Gastritis and Spleen and Stomach Damp Heat Syndrome

作　　者：江望[1] 田生望[1] 贺单[1] 陶红[1] JIANG Wang;TIAN Shengwang;HE Dan;TAO Hong(The Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China)

机构地区：[1]重庆医科大学附属第二医院,重庆400010

出　　处：《中国现代应用药学》2024年第12期1663-1670,共8页Chinese Journal of Modern Applied Pharmacy

基　　金：重庆市自然科学基金面上项目(cstc2019jcyj-msxmX0178)。

摘　　要：目的探讨三仁汤对幽门螺杆菌(Helicobacter pylori,Hp)相关性胃炎(Hp associated gastritis,HAG)脾胃湿热证大鼠消化吸收功能及Akt/NF-κB通路的影响。方法72只Wistar大鼠随机分为空白组(n=12)和造模组(n=60),以复合因素[劳倦+饮食+苦寒药+环境+生物因子(Hp菌液)]构建HAG脾胃湿热证大鼠模型。造模成功后,将造模大鼠随机分为模型组、四联疗法组(奥美拉唑2 mg·kg^(-1)+阿莫西林100 mg·kg^(-1)+克拉霉素50 mg·kg^(-1)+胶体果胶铋胶囊35 mg·kg^(-1))和三仁汤低、中、高剂量组(3、7.5、15 g·kg^(-1)),各组连续给药21 d。实验过程中观察大鼠一般情况;快速尿素酶试验检测Hp定植率;HE染色观察胃黏膜炎症情况;TUNEL检测胃黏膜组织细胞凋亡率;ELISA检测血清Ghrelin、IFN-γ、MTL、GAS、IL-4、IL-10含量;Western blotting检测胃黏膜组织caspase-3、Bax、Bcl-2、cAMP、Akt、p-Akt、NF-κB p65、p-NF-κB p65蛋白表达;Real-time PCR检测胃黏膜组织Akt、NF-κB p65 mRNA表达。结果与空白组比较,模型组大鼠黏膜组织明显损伤,Hp定植率,细胞凋亡率,胃黏膜组织caspase-3、Bax、p-Akt、p-NF-κB p65蛋白表达,胃黏膜组织Akt、NF-κB p65 mRNA表达及血清IFN-γ含量明显升高,胃黏膜组织Bcl-2蛋白表达及血清Ghrelin、MTL、GAS、IL-4、IL-10含量明显降低(P<0.01);与模型组比较,三仁汤高剂量组大鼠黏膜组织损伤明显改善,Hp定植率,细胞凋亡率,胃黏膜组织caspase-3、Bax、p-Akt、p-NF-κB p65蛋白表达,胃黏膜组织Akt、NF-κB p65 mRNA表达及血清IFN-γ含量明显降低,胃黏膜组织Bcl-2蛋白表达及血清Ghrelin、MTL、GAS、IL-4、IL-10含量明显升高(P<0.05)。结论三仁汤可改善HAG脾胃湿热证大鼠症状,抑制炎症反应及功能性消化不良,其作用机制可能与调控Akt/NF-κB通路有关。OBJECTIVE To investigate the effects of Sanren decoction on digestion and absorption function and Akt/NF-κB pathway in rats with Helicobacter pylori(Hp)-associated gastritis(HAG)and spleen and stomach damp heat syndrome.METHODS Seventy-two Wistar rats were randomly divided into blank group(n=12)and modeling group(n=60),and a rat model of HAG with combination of factors[fatigue+diet+bitter cold medicine+environment+biological factors(Hp bacterial solution)]was constructed.After successful modeling,the rats were randomly divided into model group,quadruple therapy group(omeprazole 2 mg·kg^(−1)+amoxicillin 100 mg·kg^(−1)+clarithromycin 50 mg·kg^(−1)+colloidal pectin secret capsule 35 mg·kg^(−1)),Sanren decoction low,medium,high dose groups(3,7.5,15 g·kg^(−1)),each group was continuously administered for 21 d.During the experiment,the general conditions of rats were observed;the rapid urease test was performed to detect Hp colonization rate;HE staining was performed to observe the inflammation of gastric mucosa;TUNEL was performed to detect apoptosis in gastric mucosa;the content of Ghrelin,IFN-γ,MTL,GAS,IL-4,IL-10 in serum were detected by ELISA;Western blotting was used to detect the protein expression of caspase-3,Bax,Bcl-2,cAMP,Akt,p-Akt,NF-κB p65,p-NF-κB p65 in gastric mucosa;Real-time PCR was performed to detect Akt,NF-κB p65 mRNA expression in gastric mucosal tissues.RESULTS Compared with the blank group,the mucosal tissue of rats in the model group was significantly damaged,and the Hp colonization rate,apoptosis,protein expression of caspase-3,Bax,p-Akt,p-NF-κB p65 in gastric mucosal tissue,mRNA expression of Akt,NF-κB p65 in gastric mucosal tissue and serum IFN-γcontent were significantly increased,while protein expression of Bcl-2 in gastric mucosal tissue and serum Ghrelin,MTL,GAS,IL-4 and IL-10 levels were significantly decreased(P<0.01).Compared with the model group,the mucosal tissue injury of rats in Sanren decoction high dose group was significantly improved,the Hp colonization r

关 键 词：幽门螺杆菌相关性胃炎 脾胃湿热证 三仁汤 Akt/NF-κB通路 

分 类 号：R965.2[医药卫生—药理学]