An ultra-sensitive biosensor for circulating microRNA detection with Fe single-atom enhanced cathodic luminol-O_(2) electrochemiluminescence  

作　　者：Yudie Sun Yunxiang Han Mingyue Wang Mingfu Ye Konglin Wu Kui Zhang 

机构地区：[1]School of Chemistry and Chemical Engineering,Anhui University of Technology,Ma'anshan 243032,China [2]Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science,MOE,Qingdao University of Science and Technology,Qingdao 266042,China [3]Engineering Research Center of Biofilm Water Purification and Utilization Technology of Ministry of Education,Anhui University of Technology,Ma'anshan 243032,China

出　　处：《Nano Research》2024年第9期7926-7934,共9页纳米研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.22004003);the Natural Science Foundation of Anhui Province for Distinguished Young Scholars(No.2008085J11);the Open Project Program of Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science(No.M2024-5);MOE,the Open Project of Engineering Research Center of Biofilm Water Purification and Utilization Technology of Ministry of Education(No.BWPU2023KF06);the Natural Science Research Project of Anhui Province Education Department(No.2023AH051116).

摘　　要：Circulating microRNAs(miRNAs)play a pivotal role in the occurrence and development of acute myocardial infarction(AMI),and precise detection of them holds significant clinical implications.The development of luminol-based luminophores in the field of electrochemiluminescence(ECL)for miRNA detection has been significant,while their effectiveness is hindered by the instability of co-reactant hydrogen peroxide(H_(2)O_(2)).In this work,an iron single-atom catalyst(Fe-PNC)was employed for catalyzing the luminol-O_(2) ECL system to achieve ultra-sensitive detection of myocardial miRNA.Target miRNA triggers a hybridization chain reaction(HCR),resulting in the generation of a DNA product featuring multiple sticky ends that facilitate the attachment of Fe-PNC probes to the electrode surface.The Fe-PNC catalyst exhibits high promise and efficiency for the oxygen reduction reaction(ORR)in electrochemical energy conversion systems.The resulting ECL biosensor allowed ultrasensitive detection of myocardial miRNA with a low detection limit of 0.42 fM and a wide linear range from 1 fM to 1.0 nM.Additionally,it demonstrates exceptional performance when evaluated using serum samples collected from patients with AMI.This work expands the application of single-atom catalysis in ECL sensing and introduces novel perspectives for utilizing ECL in disease diagnosis.

关 键 词：circulating miRNAs single-atom catalyst luminol-O_(2) ELECTROCHEMILUMINESCENCE hybridization chain reaction 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]