Bioinspired coacervate-based bioinks for construction of multiscale tissue engineering scaffolds  

作　　者：Zhongwei Guo Shiqiang Zhang Yilin Guo Jingjing Xia Xiao Wu Hao Hu Rongcheng Hu Fangli Huang Qiulei Gao Chun Liu Jingjiang Qiu Wei Sun 

机构地区：[1]School of Mechanics and Safety Engineering,Zhengzhou University,Zhengzhou 450001,China [2]Department of Mechanical Engineering and Mechanics,Tsinghua University,Beijing 100084,China [3]Precision Medicine Institute,Guangdong Provincial Key Laboratory of Orthopedics and Traumatology,The First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China [4]Department of Mechanical Engineering,Drexel University,Philadelphia,PA 19104,USA

出　　处：《Nano Research》2024年第9期8209-8219,共11页纳米研究（英文版）

基　　金：the projects of National Natural Science Foundation of China(No.32301209);Key R&D Projects of Henan Province(Nos.231111312400 and 241111220400).

摘　　要：Engineering hydrogels that resemble biological tissues of various lengths via conventional fabrication techniques remains challenging.Three-dimensional(3D)bioprinting has emerged as an advanced approach for constructing complex biomimetic 3D architectures,which are currently restricted by the limited number of available bioinks with high printability,biomimicry,biocompatibility,and proper mechanical properties.Inspired by ubiquitous coacervation phenomena in biology,we present a unique mineral-biopolymer coacervation strategy that enables the hierarchical assembly of nanoclay and recombinant human collagen(RHC).This system was observed to undergo a coacervation transition(liquid‒liquid phase separation)spontaneously.The formed dense phase separated from its supernatant is the coacervate of clay-RHC-rich complexes,where polymer chains are sandwiched between silicate layers.Molecular dynamics simulation was first used to verify and explore the coacervation process.Then,the coacervates were demonstrated to be potential bioinks that exhibited excellent self-supporting and shear-thinning viscoelastic properties.Through extrusion-based printing,the versatility of the bioink was demonstrated by reconstructing the key features of several biological tissues,including multilayered lattice,vascular,nose,and ear-like structures,without the need for precrosslinking operations or support baths.Furthermore,the printed scaffolds were cytocompatible,elicited minimal inflammatory responses,and promoted bone regeneration in calvarial defects.

关 键 词：bioinks COACERVATION mineral-biopolymer NANOCLAY tissue engineering scaffold 

分 类 号：R318[医药卫生—生物医学工程]