A multi-colloidal gold immunochromatography assay for rapid and simultaneous detection of 13 herbicides  

作　　者：Qianqian Lu Xinxin Xu Aihua Qu Liqiang Liu Yuzhe Li Maozhong Sun Chuanlai Xu Hua Kuang 

机构地区：[1]International Joint Research Laboratory for Biointerface and Biodetection,and School of Food Science and Technology,Jiangnan University,Wuxi 214122,China [2]China National Center for Food Safety Risk Assessment,Beijing 100021,China

出　　处：《Nano Research》2024年第9期8368-8376,共9页纳米研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.22306074 and 222360020);the National Key Research and Development Program of China(Nos.2023YFF1105003 and 2022YFA1207300);the grants from Jiangsu province(Nos.CX(22)1013,BK20212014,and M20221006).

摘　　要：Herbicide residues in agricultural products can have adverse effects on the environment and human health,therefore,there is an urgent need to establish a sensitive,rapid,and wide-ranging detection method.In this study,haptens of phenylurea herbicides(PUs)and sulfonylurea herbicides(SUs)were analyzed and designed based on computational simulation techniques,and two high-performance broad-spectrum monoclonal antibodies against PUs and SUs were prepared.On this basis,a multi-colloidal gold immunochromatography assay(multi-CGIA)was developed to simultaneously detect 13 herbicides in wheat.The visual limit of detection(vLOD)for PUs including diuron,chlortoluron,neburon,chlorbromuron,and linuron was 1-2μg/kg.The vLOD for SUs including metsulfuron methyl,ethametsulfuron-methyl,sulfometuron-methyl,tribenuron methyl,cinosulfuron,triasulfuron,chlorimuron-ethyl,and chlorsulfuron was 2-10μg/kg.The results of real sample determination indicated that the multi-CGIA is accurate,stable,and reliable,and adaptable to on-site preliminary screening of actual samples.

关 键 词：phenylurea herbicides sulfonylurea herbicides monoclonal antibody immunochromatographic assay WHEAT 

分 类 号：TQ457[化学工程—农药化工]