Molecular and structural basis of an ATPase-nuclease dualenzyme anti-phage defense complex  

作　　者：Qiyin An Yong Wang Zhenhua Tian Jie Han Jinyue Li Fumeng Liao Feiyang Yu Haiyan Zhao Yancheng Wen Heng Zhang Zengqin Deng 

机构地区：[1]Key Laboratory of Virology and Biosafety,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan,Hubei,China [2]University of Chinese Academy of Sciences,Beijing,China [3]Department of Human Anatomy,School of Basic Medical Sciences,Tianjin Medical University,Tianjin,China [4]Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Tianjin Medical University,Tianjin,China [5]State Key Laboratory of Virology,College of Life Sciences,Wuhan University,Wuhan,Hubei,China [6]Key Laboratory of Gastrointestinal Cancer(Fujian Medical University),Ministry of Education,Fuzhou,Fujian,China [7]Hubei Jiangxia Laboratory,Wuhan,Hubei,China

出　　处：《Cell Research》2024年第8期545-555,共11页细胞研究（英文版）

基　　金：supported by the National Key R&D Program of China(2022YFA1303500);the CAS Pioneer Hundred Talents Program to Z.D.

摘　　要：Coupling distinct enzymatic effectors emerges as an efficient strategy for defense against phage infection in bacterial immune responses,such as the widely studied nuclease and cyclase activities in the type III CRISPR-Cas system.However,concerted enzymatic activities in other bacterial defense systems are poorly understood.Here,we biochemically and structurally characterize a two-component defense system DUF4297–HerA,demonstrating that DUF4297–HerA confers resistance against phage infection by cooperatively cleaving dsDNA and hydrolyzing ATP.DUF4297 alone forms a dimer,and HerA alone exists as a nonplanar split spiral hexamer,both of which exhibit extremely low enzymatic activity.Interestingly,DUF4297 and HerA assemble into an approximately 1 MDa supramolecular complex,where two layers of DUF4297(6 DUF4297 molecules per layer)linked via inter-layer dimerization of neighboring DUF4297 molecules are stacked on top of the HerA hexamer.Importantly,the complex assembly promotes dimerization of DUF4297 molecules in the upper layer and enables a transition of HerA from a nonplanar hexamer to a planar hexamer,thus activating their respective enzymatic activities to abrogate phage infection.Together,our findings not only characterize a novel dual-enzyme anti-phage defense system,but also reveal a unique activation mechanism by cooperative complex assembly in bacterial immunity.

关 键 词：ENZYMATIC PLANAR neighboring 

分 类 号：O65[理学—分析化学]