SPEED:an integrated,smartphone-operated,handheld digital PCR Device for point-of-care testing  

作　　者：Haoqing Zhang Xiaocheng Liu Xinlu Wang Zhiqiang Yan Ying Xu Martina Gaňová TomášŘezníček Marie Korabečná Pavel Neuzil 

机构地区：[1]Ministry of Education Key Laboratory of Micro and Nano Systems for Aerospace,School of Mechanical Engineering,Northwestern Polytechnical University,127 West Youyi Road,Xi’an,Shaanxi 710072,PR China [2]The Key Laboratory of Biomedical Information Engineering of the Ministry of Education,School of Life Science and Technology,Xi’an Jiaotong University,Xi’an,Shaanxi 710049,PR China [3]Bioinspired Engineering and Biomechanics Center(BEBC),Xi’an Jiaotong University,Xi’an 710049,PR China [4]School of Marine Science and Technology,Northwestern Polytechnical University,Xi’an,Shaanxi 710072,PR China [5]Central European Institute of Technology,Brno University of Technology,Purkyňova 123,61300 Brno,Czech Republic [6]ITD Tech S.R.O,Osvoboditelu,1005,73581 Bohumín,Czech Republic [7]Institute of Biology and Medical Genetics,First Faculty of Medicine,Charles University and General University Hospital of Prague,Albertov 4,12800 Prague,Czech Republic

出　　处：《Microsystems & Nanoengineering》2024年第3期149-158,共10页微系统与纳米工程（英文）

基　　金：support from grant no.52150710541 provided by the National Natural Science Foundation of P.R.China;grant no.2018YFE0109000 provided by the Ministry of Science and Technology of the P.R.China;H.Z.was supported by grant no.62301412 from the Natural Science Foundation of China;grant no.2023-JCQN-0130 from the Natural Science Basic Research Program of Shaanxi Province,P.R.China;grant no.2023M732815 from the P.R.China Postdoctoral Science Foundation;grant no.2023BSHEDZZ18 from the Postdoctoral Science Foundation of Shaanxi province;M.K.was supported by grant no.LTACH19005 from the Ministry of Education,Youth;Sports of the Czech Republic and grant no.RVO-VFN 64165 from the Ministry of Health of the Czech Republic;Ministry of Health,Czech Republic-conceptual development of research organization 00064165,General University Hospital in Prague.

摘　　要：This study elaborates on the design,fabrication,and data analysis details of SPEED,a recently proposed smartphonebased digital polymerase chain reaction(dPCR)device.The dPCR chips incorporate partition diameters ranging from 50μm to 5μm,and these partitions are organized into six distinct blocks to facilitate image processing.Due to the superior thermal conductivity of Si and its potential for mass production,the dPCR chips were fabricated on a Si substrate.A temperature control system based on a high-power density Peltier element and a preheating/cooling PCR protocol user interface shortening the thermal cycle time.The optical design employs four 470 nm light-emitting diodes as light sources,with filters and mirrors effectively managing the light emitted during PCR.An algorithm is utilized for image processing and illumination nonuniformity correction including conversion to a monochromatic format,partition identification,skew correction,and the generation of an image correction mask.We validated the device using a range of deoxyribonucleic acid targets,demonstrating its potential applicability across multiple fields.Therefore,we provide guidance and verification of the design and testing of the recently proposed SPEED device.

关 键 词：CORRECTION SMART testing 

分 类 号：TN929.53[电子电信—通信与信息系统]