Acoustofluidic scanning fluorescence nanoscopy with a large field of view  

作　　者：Geonsoo Jin Neil Upreti Joseph Rich Jianping Xia Chenglong Zhao Tony Jun Huang 

机构地区：[1]Thomas Lord Department of Mechanical Engineering and Materials Science,Duke University,Durham,NC 27708,USA [2]Department of Biomedical Engineering,Duke University,Durham,NC 27708,USA [3]The MITRE Corporation,McLean,VA 22102,USA

出　　处：《Microsystems & Nanoengineering》2024年第3期159-167,共9页微系统与纳米工程（英文）

基　　金：support from the National Institutes of Health(R01GM143439,R01HD103727,UH3TR002978,and U18TR003778);the National Science Foundation(CMMI-2104295);a National Science Foundation Graduate Research Fellowship under Grant no.2139754.

摘　　要：Large-field nanoscale fluorescence imaging is invaluable for many applications,such as imaging subcellular structures,visualizing protein interactions,and high-resolution tissue imaging.Unfortunately,conventional fluorescence microscopy requires a trade-off between resolution and field of view due to the nature of the optics used to form the image.To overcome this barrier,we developed an acoustofluidic scanning fluorescence nanoscope that simultaneously achieves superior resolution,a large field of view,and strong fluorescent signals.The acoustofluidic scanning fluorescence nanoscope utilizes the superresolution capabilities of microspheres that are controlled by a programmable acoustofluidic device for rapid fluorescence enhancement and imaging.The acoustofluidic scanning fluorescence nanoscope resolves structures that cannot be resolved with conventional fluorescence microscopes with the same objective lens and enhances the fluorescent signal by a factor of~5 without altering the field of view of the image.The improved resolution realized with enhanced fluorescent signals and the large field of view achieved via acoustofluidic scanning fluorescence nanoscopy provides a powerful tool for versatile nanoscale fluorescence imaging for researchers in the fields of medicine,biology,biophysics,and biomedical engineering.

关 键 词：FIELD RESOLUTION utilize 

分 类 号：O62[理学—有机化学]