An acoustofluidic device for the automated separation of platelet-reduced plasma from whole blood  

作　　者：Zhehan Ma Jianping Xia Neil Upreti Emeraghi David Joseph Rufo Yuyang Gu Kaichun Yang Shujie Yang Xiangchen Xu Jean Kwun Eileen Chambers Tony Jun Huang 

机构地区：[1]Thomas Lord Department of Mechanical Engineering and Materials Science,Duke University,Durham,NC,USA [2]Department of Biomedical Engineering,Duke University,Durham,NC,USA [3]Department of Pediatrics,Duke University,Durham,NC,USA [4]Duke Transplant Center,Department of Surgery,Duke University School of Medicine,Durham,NC,USA

出　　处：《Microsystems & Nanoengineering》2024年第3期293-303,共11页微系统与纳米工程（英文）

基　　金：support from the National Institutes of Health(R01GM132603,R01GM141055,R44HL140800,and R21HD102790);Z.M.acknowledges the financial support from the China Scholarship Council;E.C.was funded by the Hartwell Foundation.

摘　　要：Separating plasma from whole blood is an important sample processing technique required for fundamental biomedical research,medical diagnostics,and therapeutic applications.Traditional protocols for plasma isolation require multiple centrifugation steps or multiunit microfluidic processing to sequentially remove large red blood cells(RBCs)and white blood cells(WBCs),followed by the removal of small platelets.Here,we present an acoustofluidic platform capable of efficiently removing RBCs,WBCs,and platelets from whole blood in a single step.By leveraging differences in the acoustic impedances of fluids,our device generates significantly greater forces on suspended particles than conventional microfluidic approaches,enabling the removal of both large blood cells and smaller platelets in a single unit.As a result,undiluted human whole blood can be processed by our device to remove both blood cells and platelets(>90%)at low voltages(25 Vpp).The ability to successfully remove blood cells and platelets from plasma without altering the properties of the proteins and antibodies present creates numerous potential applications for our platform in biomedical research,as well as plasma-based diagnostics and therapeutics.Furthermore,the microfluidic nature of our device offers advantages such as portability,cost efficiency,and the ability to process small-volume samples.

关 键 词：REMOVE SEPARATION removing 

分 类 号：R318[医药卫生—生物医学工程]