Strain sensor on a chip for quantifying the magnitudes of tensile stress on cells  

作　　者：Yuyin Zhang Yue Wang Hongze Yin Jiahao Wang Na Liu Songyi Zhong Long Li Quan Zhang Tao Yue 

机构地区：[1]School of Mechatronics Engineering and Automation,Shanghai University,Shanghai,China [2]School of Future Technology,Shanghai University,Shanghai,China [3]Key Laboratory of Advanced Manufacturing Technology of Zhejiang Province,School of Mechanical Engineering,Zhejiang University,Hangzhou,China [4]Shanghai Key Laboratory of Intelligent Manufacturing and Robotics,Shanghai University,Shanghai,China [5]Shanghai Institute of Intelligent Science and Technology,Tongji University,Shanghai,China

出　　处：《Microsystems & Nanoengineering》2024年第3期305-315,共11页微系统与纳米工程（英文）

基　　金：supported by grants from the National Natural Science Foundation of China(Nos.62373235,62303290,and 62073208);the Shanghai Science and Technology Committee Natural Science Foundation(No.23ZR1423700);the Shanghai Municipal Education Innovation Project 2021-09-E00113.

摘　　要：During cardiac development,mechanotransduction from the in vivo microenvironment modulates cardiomyocyte growth in terms of the number,area,and arrangement heterogeneity.However,the response of cells to different degrees of mechanical stimuli is unclear.Organ-on-a-chip,as a platform for investigating mechanical stress stimuli in cellular mimicry of the in vivo microenvironment,is limited by the lack of ability to accurately quantify externally induced stimuli.However,previous technology lacks the integration of external stimuli and feedback sensors in microfluidic platforms to obtain and apply precise amounts of external stimuli.Here,we designed a cell stretching platform with an in-situ sensor.The in-situ liquid metal sensors can accurately measure the mechanical stimulation caused by the deformation of the vacuum cavity exerted on cells.The platform was applied to human cardiomyocytes(AC16)under cyclic strain(5%,10%,15%,20 and 25%),and we found that cyclic strain promoted cell growth induced the arrangement of cells on the membrane to gradually unify,and stabilized the cells at 15%amplitude,which was even more effective after 3 days of culture.The platform’s precise control and measurement of mechanical forces can be used to establish more accurate in vitro microenvironmental models for disease modeling and therapeutic research.

关 键 词：STRESS DEFORMATION arrangement 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]