TaNAC069转基因抗叶锈病小麦种质的创制与分析  

Development and assessment of wheat lines resistant to leaf rust by genetic transformation of TaNAC069

作　　者：秦臻 崔钟池 耿怀民 刘长山 王海燕[1] 刘大群[1] QIN Zhen;CUI Zhongchi;GENG Huaimin;LIU Changshan;WANG Haiyan;LIU Daqun(College of Plant Protection,Hebei Agricultural University/Biological Control Technological Innovation Center of Plant Diseases and Plant Pests of Hebei Province,Baoding 071000,China)

机构地区：[1]河北农业大学植物保护学院/河北省农作物病虫害生物防治技术创新中心,河北保定071000

出　　处：《河北农业大学学报》2024年第4期10-16,共7页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金资助项目(C2020204028).

摘　　要：转录因子是植物中重要的一类抗病相关蛋白,其中NAC(NAM、ATAF1/2和CUC)类转录因子在植物应对生物胁迫和非生物胁迫方面起着重要作用。本研究前期获得了1个小麦(Triticum aestivum L.)NAC类转录因子TaNAC069,并初步证明其参与小麦抗叶锈病防御反应。本研究将TaNAC069基因构建到过表达载体pLGY-02中,利用农杆菌(Agrobacterium)介导的遗传转化法将pLGY-02-TaNAC069重组载体导入小麦感叶锈病农家品种‘JW’中,获得11个TaNAC069转基因T0株系。对T_(1)~T_(4)代植株进行PCR检测,明确TaNAC069已转入11个小麦株系中,并能稳定遗传;实时荧光定量PCR(Quantitative real-time PCR,qPCR)检测明确T1代11个转基因株系中TaNAC069基因的表达量显著高于受体材料‘JW’;对TaNAC069转基因小麦进行叶锈病抗性鉴定,TaNAC069转基因小麦叶锈病的发病程度比‘JW’轻,孢子量较少,表明TaNAC069基因过表达提高了转基因小麦对叶锈病的抗性。本研究明确了TaNAC069基因在小麦防御叶锈病过程中起正向调控作用,为解析TaNAC069的抗叶锈病机制奠定了基础。Transcription factors are important disease resistance related proteins in plants.The NAC(NAM,ATAF1/2 and CUC)transcription factor is a unique type in plants,which plays an essential role in plant response to both biotic and abiotic stresses.In our previous study,a wheat(Triticum aestivum L.)NAC transcription factor TaNAC069 was found to be involved in resistance to Puccinia triticina Erikss.(Pt).In this study,TaNAC069 was constructed into the overexpression vector pLGY-02.Then,the recombinant vector pLGY-02-TaNAC069 was transformed into susceptible material‘JW’by Agrobacterium-mediated genetic transformation,resulting in 11 stable transgenic wheat lines.PCR validation was performed on the T_(1)~T_(4)generation plants of the TaNAC069 transgenic wheat,confirming that TaNAC069 has been transferred into 11 wheat lines with genetic stability.Quantitative real-time PCR(qPCR)analysis confirmed that the expression of TaNAC069 gene in 11 positive transgenic plants was higher than that in‘JW’.The incidence of wheat leaf rust in the TaNAC069 transgenic lines was milder than that of‘JW’with lower spore count,indicating that overexpression of the TaNAC069 gene enhanced the wheat resistance ability against wheat leaf rust.All these results suggested that TaNAC069 played a positive role in defense against wheat leaf rust,and it will be useful to discover the mechanism of TaNAC069 in wheat resistance to leaf rust.

关 键 词：小麦 叶锈菌 NAC转录因子 转基因 抗病性 

分 类 号：S432.1[农业科学—植物病理学]