雄性大鼠高原环境暴露生殖系统损伤研究  

Reproductive damage of male rats exposed to plateau environment

作　　者：卜子涵 周浩 李家豪 张斌 张春雷 常德辉 BU Zihan;ZHOU Hao;LI Jiahao;ZHANG Bin;ZHANG Chunlei;CHANG Dehui(Department of Urology,The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army,Lanzhou,Gansu 730050,China;Gansu Provincial Key Laboratory of Stem Cell and Gene Drugs,The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army,Lanzhou,Gansu 730050,China;First Clinical School of Gansu University of Traditional Chinese Medicine,Lanzhou,Gansu 730000,China)

机构地区：[1]中国人民解放军联勤保障部队第九四〇医院泌尿外科,甘肃兰州730050 [2]中国人民解放军联勤保障部队第九四〇医院甘肃省干细胞与基因药物重点实验室,甘肃兰州730050 [3]甘肃中医药大学第一临床医学院,甘肃兰州730000

出　　处：《预防医学》2024年第8期727-730,736,共5页China Preventive Medicine Journal

基　　金：军队保健专项科研课题(21BJZ43);军队计生专项科研课题(21JSZ13);甘肃省自然科学基金项目(22JR5RA001;23JR⁃RA531);国家重大需求培育专项(31920240072)。

摘　　要：目的探究高原环境暴露对雄性大鼠生殖系统的影响,为高原环境下生殖损伤机制研究提供依据。方法60只SPF级12周龄雄性Wistar大鼠随机分为平原组、高原1 d组、高原3 d组、高原7 d组、高原14 d组和高原28 d组。平原组大鼠在正常环境下常规饲养28 d,各高原组大鼠置于模拟高原低压低氧动物实验舱暴露相应时间。各组大鼠完成饲养时间后麻醉处死,取睾丸组织和腹主动脉血。检测睾丸指数和精子质量;分析睾丸组织形态和细胞器形态;测定睾丸组织丙二醛(MDA)、超氧化物歧化酶(SOD)、活性氧(ROS),以及血清促卵泡激素(FSH)、黄体生成素(LH)和睾酮(T)。结果与平原组比较,高原14 d组大鼠睾丸指数、相对精子计数、精子活力和精子前向运动率降低;睾丸组织形态异常较明显,约翰逊评分较低;线粒体破裂,基质明显变浅,嵴大量断裂、缺失;睾丸组织MDA、ROS水平升高;血清FSH、LH水平降低,T水平升高(均P<0.05)。与高原14 d组比较,高原28 d组大鼠相对精子计数、精子活力和精子前向运动率升高;线粒体仍肿胀,但未见线粒体破裂;睾丸组织MDA、ROS水平降低,SOD水平升高;血清FSH、LH水平升高,T水平降低(均P<0.05)。结论高原环境可引起雄性大鼠睾丸指数、精子质量下降,线粒体功能受损,诱发氧化应激,从而影响雄性大鼠生殖系统;但随着暴露时间增加,大鼠生殖系统出现自我修复现象。Objective To investigate the effects of plateau environment exposure on the reproductive system of male rats,so as to provide the reference for mechanisms of reproductive damage in plateau environment.Methods Sixty SPF-grade 12-week-old male Wistar rats were randomly divided into the plain-exposed group,the 1 day-,3 day-,7 day-,14 day-and 28 day-plateau-exposed groups.The rats in the plain-exposed group were raised under normal conditions for 28 days,while the rats in the plateau-exposed groups were raised in a simulated high-altitude plateau chamber.After the completion of the designated feeding periods,the rats were sacrificed under anesthesia,and testicular tissue and abdominal aortic blood were collected to detect the testicular index and evaluate sperm quality.Histological and cellular morphologies of the testicular tissue were analyzed.Additionally,the levels of malondialdehyde(MDA),superoxide dismutase(SOD)and reactive oxygen(ROS)in the testicular tissue were determined,along with serum levels of follicle-stimulating hormone(FSH),luteinizing hormone(LH)and testosterone(T).Results Compared with the plain exposed group,the rats in the 14 day-plateau-exposed group showed a decrease in the testicular index,relative sperm count,sperm viability and forward motility rate;more abnormalities in testicular tissue morphology,resulting in lower Johnson score;ruptures in mitochondria,significantly paler matrix,and more fragmented and missing cristae;increased MDA and ROS levels;decreased FSH and LH levels and an increased T level(all P<0.05).Compared with the 14 day-plateauexposed group,the rats in the 28 day-plateau-exposed group showed an increase in relative sperm count,sperm viability and forward motility rate;swollen mitochondria but no ruptures;decreased MDA and ROS levels,increased SOD levels;increased FSH and LH levels,and a decreased T level(all P<0.05).Conclusions Plateau environment may cause a decrease in testicular index and sperm quality,impair mitochondrial function,induce oxidative stress,and thus affec

关 键 词：高原 生殖系统 睾丸 氧化应激 性激素 

分 类 号：R594.3[医药卫生—内科学]