响应面法优化肉桂子总有机酸提取工艺及其抗氧化活性研究  

Optimization of Total Organic Acid Extraction Process and Antioxidant Activity of Fructus Cinnamomi Cassiae Immaturi by Response Surface Methodology

作　　者：黄梦 刘宏炳[1,2] 杨珍 王彦顺 燕雪花 HUANG Meng;LIU Hongbing;YANG Zhen;WANG Yanshun;YAN Xuehua(School of Traditional Chinese Medicine,Xinjiang Medical University,Xinjiang Urumqi 830017,China;Key Laboratory of Famous Medical Formulas and Characteristic Formulas in Xinjiang,Xinjiang Urumqi 830017,China)

机构地区：[1]新疆医科大学中医学院,新疆乌鲁木齐830017 [2]新疆维吾尔自治区新疆名医名方与特色方剂学重点实验室,新疆乌鲁木齐830017

出　　处：《饲料工业》2024年第16期132-139,共8页Feed Industry

基　　金：新疆维吾尔自治区药品监督管理局科研项目[20210012]

摘　　要：采用响应面法优化肉桂子中总有机酸的提取工艺并研究其体外抗氧化活性。根据Box-Behnken Design设计原理,分别考察乙醇浓度、料液比、超声功率、超声时长对肉桂子总有机酸得率的影响。以肉桂子总有机酸得率为响应值,通过响应面法确定肉桂子总有机酸的最佳提取工艺。采用DPPH法、铁离子还原能力评价肉桂子总有机酸抗氧化能力。结果表明,单因素影响顺序依次为料液比>乙醇浓度>超声时长。肉桂子中总有机酸的最优提取工艺为:乙醇浓度59%、料液比1:60(g/mL)、超声功率350 W,超声时长49 min,得到的总有机酸的平均得率9.77%。VC及S-1肉桂子的IC50分别为0.01119、0.01437 mg/mL。说明该提取工艺稳定、可行、可靠;肉桂子具有一定抗氧化能力。To optimize the extraction process of total organic acids from Fructus Cinnamomi cassiae immaturi using response surface methodology and study their in vitro antioxidant activity.Based on the design principle of Box Behnken Design,the effects of ethanol concentration,solid-liquid ratio,ultrasound power,and ultrasound duration on the total organic acid yield of cinnamon seeds were investigated.Using the yield of total organic acids from Fructus Cinnamomi cassiae immaturi as the response value,the optimal extraction process for total organic acids from Fructus Cinnamomi cassiae immaturi was determined through response surface optimization.DPPH method and iron ion reduction ability were used to evaluate the total organic acid antioxidant capacity of Fructus Cinnamomi cassiae immaturi.The results indicate that the order of single factor influence is material liquid ratio>ethanol concentration>ultrasonic time.The optimal extraction process for total organic acids in Fructus Cinnamomi cassiae immaturi is as follows:ethanol concentration 59%,solid-liquid ratio 1∶60(g/mL),ultrasonic power 350 W,ultrasonic time 49 minutes,and the average content of total organic acids obtained is 9.77%.The IC50 of VC and S-1 were 0.01119 mg/mL and 0.01437 mg/mL,respectively.This extraction process is stable,feasible,and reliable;It has certain antioxidant capacity.

关 键 词：肉桂子 响应曲面法 总有机酸 含量测定 抗氧化 

分 类 号：S816.32[农业科学—饲料科学]