诃子提取物对脂多糖刺激肉鸡肠道和肝脏损伤的影响  

Effect of Chebuli Extract on Intestinal and Liver Damage Induced by Lipopolysaccharide in Broilers

作　　者：张晓涵 孙岚源 宋转 侯永清[1] 吴涛[1] ZHANG Xiaohan;SUN Lanyuan;SONG Zhuan;HOU Yongqing;WU Tao(Hubei Provincial Key Laboratory of Animal Nutrition and Feed Science,Engineering Research Center of the Ministry of Education,Agricultural and Sideline Product Protein Feed Resources,Wuhan Polytechnic University,Wuhan 430023,China)

机构地区：[1]武汉轻工大学,农副产品蛋白质饲料资源教育部工程研究中心,动物营养与饲料科学湖北省重点实验室,武汉430023

出　　处：《中国畜牧兽医》2024年第8期3267-3278,共12页China Animal Husbandry & Veterinary Medicine

基　　金：湖北省重点研发计划项目(2022BBA0014)。

摘　　要：[目的]研究诃子提取物(chebuli extract,CE)对脂多糖(lipopolysaccharide,LPS)刺激肉鸡肠道形态、肠道和肝脏炎症反应的影响,以期为功能性饲料添加剂的开发提供理论依据。[方法]采用双因素试验设计,选取40只健康的1日龄罗斯308肉鸡,随机分为2组:对照组和CE处理组,每组5个重复,每个重复4只鸡。对照组肉鸡饲喂基础饲粮,CE处理组肉鸡在基础饲粮基础上添加1000 mg/kg诃子提取物。试验期29 d。第29天,从每个重复中取2只肉鸡腹腔注射1 mg/kg BW LPS,另2只肉鸡腹腔注射等体积生理盐水。注射LPS 3 h后屠宰肉鸡,采集肝脏、脾脏、胸腺和法氏囊并称重,计算脏器系数,并检测肠道组织形态、肠道屏障功能相关基因、肠道和肝脏炎症反应相关基因的表达水平。[结果]①与未经LPS刺激肉鸡相比,LPS刺激显著降低了肉鸡十二指肠的绒毛高度、隐窝深度、绒毛高度/隐窝深度和绒毛面积,以及空肠绒毛高度、回肠绒毛面积(P<0.05);添加诃子提取物显著降低了肉鸡空肠的隐窝深度,显著升高了空肠和回肠的绒毛高度、绒毛高度/隐窝深度、绒毛面积(P<0.05)。在LPS刺激下,添加诃子提取物显著升高了肉鸡十二指肠、空肠和回肠的绒毛高度,以及十二指肠和回肠的绒毛高度/隐窝深度、空肠和回肠的绒毛面积(P<0.05)。②与未经LPS刺激肉鸡相比,添加诃子提取物显著提高了肉鸡黏蛋白2(Mucin-2)、闭锁小带蛋白1(zonula occluden-1,ZO-1)、闭锁蛋白(Occludin)基因表达量(P<0.05)。在LPS刺激下,添加诃子提取物显著上调了肉鸡Claudin-1、Mucin-2、ZO-1基因表达量(P<0.05)。③与未经LPS刺激肉鸡相比,LPS刺激显著提高了肉鸡肝脏基质金属蛋白酶9(matrix metallopeptidase 9,MMP9)、白细胞介素-1β(interleukin-1β,IL-1β)、IL-8和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)基因表达量(P<0.05);添加诃子提取物显著下调了肉鸡肝脏MMP 9、IL-1β和IL-8基因表[Objective]This study was conducted to investigate the effect of chebuli extract(CE)on the intestinal morphology,intestinal and liver inflammatory responses of lipopolysaccharide(LPS)-stimulated broilers,so as to provide a theoretical basis for the development of functional feed additives.[Method]This experiment adopted a two-factor experimental design.40 healthy 1-day-old Ross 308 broilers were selected and randomly divided into control and CE treatment groups.Each group had 5 replicates with 4 broilers in each replicate.The broilers in control group were fed basal diet,and broilers in CE treatment group was supplemented with 1000 mg/kg CE on the basis of basal diet.The experimental period was 29 days.On the 29th day,2 broilers in each replicate were intraperitoneally injected with 1 mg/kg BW LPS,and the other 2 broilers were intraperitoneally injected with normal saline.Slaughter sampling was performed 3 h after LPS injection.The liver,spleen,thymus and bursa of Fabricius of broilers were weighed to calculate organ coefficients.Intestinal tissue morphology,intestinal barrier-related genes,and intestinal and liver inflammation-related genes of broilers were measured.[Result]①Compared with broilers without LPS stimulation,LPS stimulation significantly reduced villus height,crypt depth,villus height/crypt depth and villus area of duodenum,and jejunum villus height and ileum villus area in broilers(P<0.05);Adding CE significantly reduced the jejunum crypt depth,and significantly increased villus height,villus height/crypt depth and villus area of jejunum and ileum(P<0.05).Compared with LPS-stimulated broilers,adding CE significantly increased villus height of duodenum,jejunum and ileum,villus height/crypt depth of duodenum and ileum,and villus area of jejunum and ileum(P<0.05).②Compared with broilers without LPS stimulation,adding CE significantly increased the expression of Mucin-2,zonula occludin 1(ZO-1)and Occludin genes in broilers(P<0.05).Compared with LPS-stimulated broilers,adding CE significantly increa

关 键 词：诃子提取物 脂多糖 肉鸡 肝脏 肠道健康 

分 类 号：S816.7[农业科学—饲料科学]