载铜纳米粒鼻用原位凝胶体外释放度研究  

In Vitro Release of Copper-loaded Nanoparticles from Nasal in situ Gels

作　　者：曹雅 仇丽颖 CAO Ya;QIU Li-ying(School of Pharmacy,Southwest Minzu University,Chengdu 610225,China)

机构地区：[1]西南民族大学药学院,成都610225

出　　处：《科学技术与工程》2024年第22期9302-9308,共7页Science Technology and Engineering

基　　金：西南民族大学中央高校基本科研业务费专项资金项目(2018NQN08)。

摘　　要：为研究载铜纳米粒鼻用原位凝胶(TETA-Cu-NPs-gel)中三乙烯四胺铜(TETA-Cu)的体外释放规律,通过透析法研究TETA-Cu的体外释放度,以0.1 mol/mL磷酸盐缓冲液(PBS,pH=7.4)为释放介质,并用Origin软件对释药数据进行模型拟合。采用高效液相色谱法检测TETA-Cu在溶出介质中的释放水平,以Swell Chromplus^(TM)C_(18)(4.6 mm×150 mm,5μm)为色谱柱,流动相A为pH=3.0磷酸盐缓冲液,流动相B为甲醇(A∶B=90∶10),流速1.0 mL/min,检测波长为260 nm。结果表明,TETA-Cu在0.05~1 mg/mL范围内线性关系良好,高、中、低三个浓度的回收率在99.9%~101.0%,RSD均小于2%;与TETA-Cu溶液相比,TETA-Cu-NPs-gel释药速率缓慢,符合一级释药动力学方程。可见,本文研究所建立的TETA-Cu-NPs-gel体外释放度测定方法准确、简便可行。TETA-Cu-NPs-gel具有缓释的作用。In order to study the in vitro release pattern of copper triethylenetetramine(TETA-Cu)from copper-loaded nanoparticles in nasal in situ gels(TETA-Cu-NPs-gel).Dialysis was used to investigate in vitro release.0.1 mol/mL phosphate buffer solution(PBS,pH=7.4)was used as the release medium and the model was fitted to the release data using Origin software.HPLC(high performance liquid chromatography)was used to investigate the release level of TETA-Cu in dissolution media.The chromatographic column was Swell Chromplus^(TM)C_(18)(4.6 mm×150 mm,5μm),mobile phase A was pH=3.0 phosphate buffer,and mobile phase B was methanol(A∶B=90∶10).The flow rate was 1.0 mL/min,the detection wavelength was 260 nm,Intake quantity 20μL.The results show that TETA-Cu showed good linearity in the range of 0.05~1 mg/mL.The recoveries of the high,medium,and low concentrations are in the range of 99.9%~101.0%,with the RSDs of less than 2%.Compared with the TETA-Cu solution,the rate of drug release of TETA-Cu-NPs-gel is slow,which conformed to the kinetic equation of the first level of drug release.It is concluded that the in vitro release assay of TETA-Cu-NPs-gel established in this study is accurate,simple,and feasible.TETA-Cu-NPs-gel has a slow-release effect.

关 键 词：三乙烯四胺铜 铜补充剂 高效液相色谱 体外释放度 离子型凝胶 

分 类 号：R944.9[医药卫生—药剂学]