甘肃省辣椒炭疽病病原菌鉴定  

Identification of Pathogen Causing Anthracnose of Pepper(Capsicum annuum L.)in Gansu Province

作　　者：荆卓琼[1,2] 郭致杰 孙倩[1,2] 徐生军 JING Zhuoqiong;GUO Zhijie;SUN Qian;XU Shengjun(Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070;Scientific Observing and Experimental Station of Crop Pests in Tianshui,Ministry of Agriculture and Rural Affairs,Tianshui,Gansu 741200)

机构地区：[1]甘肃省农业科学院植物保护研究所,甘肃兰州730070 [2]农业农村部天水作物有害生物科学观测实验站,甘肃天水741200

出　　处：《北方园艺》2024年第15期1-7,共7页Northern Horticulture

基　　金：国家重点研发计划资助项目(2021YFD1400202);兰州市科技计划资助项目(2021-1-174);国家自然科学基金资助项目(32060634);甘肃省农业科学院农业科技创新专项资助项目(2021GAAS54);甘肃省农业科学院科技成果转化资助项目(2021GAAS-CGZH01)。

摘　　要：以从甘肃省兰州市安宁区采集疑似辣椒炭疽病的发病辣椒果实为试材,采用组织分离法分离病原菌,通过构建多基因联合系统发育树,结合形态学和柯赫氏法则鉴定兰州市辣椒炭疽病病原真菌种类,以期为该病的诊断和防控提供参考依据。结果表明:从具有典型症状的病果上分离得到13株具有相同培养性状的菌株,柯赫氏法则明确代表菌株CA1902为辣椒炭疽病的致病菌;通过ITS、TUB2、ACT、CHS-1、HIS3和GAPDH构建多基因联合系统发育树,该菌株与松针炭疽菌Colletotrichum fioriniae聚在同一分支,且支持率为100%。综合可知,甘肃省兰州市安宁区辣椒炭疽病病原菌种类为松针炭疽菌C.fioriniae,是该种引起甘肃地区辣椒炭疽病的首次报道。考虑到辣椒的经济价值,可依据该鉴定结果制定有效的病害防控措施。Taking diseased chili fruits with typical anthracnose symptoms collected from Anning district,Lanzhou city,Gansu Province as the test materials,taking tissue isolation method to isolate pathogens,a multi gene combined phylogenetic tree,and combining morphology and Koch's rule were constructed to identify the fungal species causing pepper anthracnose in Lanzhou city,in order to provide reference for the diagnosis and prevention and control of this disease.The results showed that 13 strains with the same culture characteristics were obtained from typical anthracnose pepper fruits.The assay of Koch's postulates confirmed that CA1902 was the causal agent of pepper anthracnose that were identified as Colletotrichum fioriniae by morphology and multilocus phylogenies from combined datasets of ITS,TUB2,ACT,CHS1,HIS3 and GAPDH.The isolate CA1902 clustered with C.fioriniae,having 10o%bootstrap support.According to the above,the pathogen causing anthracnose of pepper(Capsicum annuum L.)was Colletotrichum fioriniae in Anning district,in Lanzhou,Gansu Province.This study was the first report of C.fioriniae causing anthracnose on pepper in Gansu Province,China.Considering the economic value of pepper,this identification maight aid in the selection of appropriate management tactics for this disease.

关 键 词：辣椒炭疽病 分离鉴定 多基因联合系统发育分析 松针炭疽菌 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]