石斛兰杂交后代SRAP鉴定与遗传多样性分析  

Identification and Genetic Diversity Analysis of Hybrid Progenies of Dendrobium Based on SRAP Markers

作　　者：林榕燕 陈艺荃 孔兰 钟淮钦[1] 林兵[1] 叶秀仙[1] LIN Rongyan;CHEN Yiquan;KONG Lan;ZHONG Huaiqin;LIN Bing;YE Xiuxian(Crop Research Institute(Fujian Germplasm Resource Center),Fujian Academy of Agricultural Sciences/Fujian Engineering Research Center for Characteristic Floriculture,Fuzhou,Fujian 350013)

机构地区：[1]福建省农业科学院作物研究所(福建省种质资源中心),福建省特色花卉工程技术研究中心,福建福州350013

出　　处：《北方园艺》2024年第15期46-52,共7页Northern Horticulture

基　　金：福建省省属公益类科研院所资助项目(2022R1031009);福建省农业科学院对外合作资助项目(DWHZ-2023-06);福建省农业科学院自由探索科技创新资助项目(ZYTS2023003);福建省农业科学院科技创新团队建设资助项目(CXTD2021010-2);福建省种业工程资助项目(ZYCX-LY-202102)。

摘　　要：以麝香石斛、檀香石斛及麝香石斛×檀香石斛的100个杂交后代株系为试材,采用SRAP分子标记的方法,研究了杂交后代的真实性及亲本与杂交后代群体的遗传多样性,以期为加速石斛兰新品种的选育提供参考依据。结果表明:综合6对SRAP引物组合的扩增情况,共鉴定出100个杂交后代为真杂种,鉴定效率达100%;6对SRAP引物组合的多态性比例达97.26%,多态性信息含量均值为0.85;由聚类分析可知,92%的杂交后代在亲缘关系上与父本聚为一类;由遗传相似系数分析可知,杂交后代与父本间的遗传相似系数平均值大于母本。综上所述,供试的麝香石斛×檀香石斛杂交后代均为真杂种,且表现为偏父本遗传,SRAP分子标记可作为石斛兰杂交后代早期鉴定的有效手段。One hundred hybrid progenies of Dendrobium progenies,D.anosmum and D.porishi × D.anosmum were used as the test materials,the authenticity of the hybrid progenies was identified by SRAP molecular markers,their genetic diversity was also analyzed,in order to provide reference for accelerating the breeding process of new Dendrobium varieties.The results showed that 100 hybrid progenies were identified as true hybrids,with an identification efficiency of 100%,based on the amplification of 6 pairs of SRAP primer combinations.The polymorphic ratio of the 6 pairs of SRAP primer combinations was 97.26%,and the average of polymorphism information content was 0.85.According to the cluster diagram,92%of the hybrid offspring were clustered with the paternal parent in terms of parentage.According to the box-plot of genetic similarity coefficient analysis,the average genetic similarity coefficient between the hybrid offspring and the male parent was greater than that of the female parent,which indicated that the offspring of the hybrid combination were genetically more biased towards the male parent.In conclusion,the tested hybrid offspring of D.parishiiXD.anosmum were all true hybrids and showed partial paternal inheritance.SRAP molecular markers could be used as an effective means for early identification of hybrid offspring in Dendrobium.

关 键 词：石斛兰 SRAP标记 杂交后代鉴定 遗传多样性 

分 类 号：S682.31[农业科学—观赏园艺]