“四季”青绿苔草多倍体诱导及鉴定  

Polyploid Induction and Identification of Carex breviculmis ‘Siji'

作　　者：贾明 董双慧 潮洛濛 范希峰[1] 武菊英[1] 岳跃森[1] JIA Ming;DONG Shuanghui;CHAO Luomeng;FAN Xifeng;WU Juying;YUE Yuesen(Institute of Grassland,Flowers and Landscape Ecology,Beijing Academy of Agriculture and Forestry Sciences/North China Key Laboratory of Urban Agriculture,Ministry of Agriculture and Rural Affairs,Beijing 100097;School of Ecology and Environment,Inner Mongolia University,Hohhot,Inner Mongolia 01021)

机构地区：[1]北京市农林科学院草业花卉与景观生态研究所,农业农村部华北都市农业重点实验室,北京100097 [2]内蒙古大学生态与环境学院,内蒙古呼和浩特010021

出　　处：《北方园艺》2024年第15期52-59,共8页Northern Horticulture

基　　金：北京市农林科学院科技创新专项资助项目(KJCX20230811,KJCX20230119);现代农业产业技术体系北京市创新团队资助项目(BAIC09-2023)。

摘　　要：以“四季”青绿苔草的胚性愈伤组织为试验材料,选用秋水仙素为诱导剂,采用秋水仙素浸泡处理法进行诱变处理,研究了不同的秋水仙素浓度和不同的处理时间下诱导“四季”青绿苔草多倍体的最佳条件。通过流式细胞术、根尖染色体计数的方法对加倍植株进行倍性鉴定,并进行叶片气孔特征和植株表型特征分析,以期为“四季”青绿苔草的多倍体诱导及苔草新品种培育提供参考依据。结果表明:在秋水仙素浓度为0.10%,处理时间为48 h时诱导率最高,该条件下的诱导率可达65%。通过植株表型与气孔特征比较,加倍后的植株与二倍体植株有明显的差异,表现为叶型宽阔、株高降低、气孔变大等特点。Taking the embryogenic callus of Carer breviculmis‘Siji'as the test material,selecting colchicine as the inducer,and using the colchicine soaking method for mutagenesis treatment.The optimal conditions for inducing polyploidy of Carer breviculmis‘Siji'were under different colchicine concentrations and different treatments times.The ploidy of doubled plants was identified by flow cytometry and root tip chromosome counting,and leaf stomatal characteristics and plant phenotypic characteristics were analyzed,in order to provide reference for the polyploid induction of Carea breviculmis‘Siji'and the cultivation of new Carer varieties.The results showed that the induction rate was the highest when the colchicine concentration was 0.1o%and the treatment time was 48 hours,and the induction rate under these conditions could reach 65%.Comparing the plant phenotype and stomatal characteristics,it was found that the doubled plants were significantly different from the diploid plants,which were characterized by broad leaf shape,reduced plant height,and enlarged stomatal pores.

关 键 词：“四季”青绿苔草 多倍体诱导 秋水仙素 倍性鉴定 

分 类 号：S688.4[农业科学—观赏园艺]