阳虚体质膝骨性关节炎患者Notch1、Notch2表达与雌二醇水平的相关性研究  

Correlational Study on the Expressions of Notch1 and Notch2 in Knee Osteoarthritis Patients of Yang Deficiency Constitution and the Levels of Estradiol

作　　者：魏长浩 李盛华[2] 苏耀辉 王晓萍[2] 吉星 王玉磊 黄克松 郑昊天 高向明 周明旺[2] WEI Changhao;LI Shenghua;SU Yaohui;WANG Xiaoping;JI Xing;WANG Yulei;HUANG Kesong;ZHENG Haotian;GAO Xiangming;ZHOU Mingwang(Gansu University of Chinese Medicine,Lanzhou 730000,China;Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃省中医院,甘肃兰州730050

出　　处：《西部中医药》2024年第8期7-11,共5页Western Journal of Traditional Chinese Medicine

基　　金：甘肃省科技计划项目(18JR2FA009);中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者项目(国中医药人教[2018]12号);国家中医药管理局全国中医学术流派传承工作室建设项目(LP28061);甘肃中医药大学研究生创新基金项目(CY2020-03)

摘　　要：目的:探讨阳虚体质膝骨性关节炎(knee osteoarthritis,KOA)患者雌二醇(estradiol,E2)水平对Notch1、Notch2信号通路的影响及相关性研究。方法:选取60例KOA患者,根据体质分型分为阳虚体质组与非阳虚体质组,每组30例。空腹采集外周静脉血,采用电化学发光法检测E2浓度;采用q-PCR与Western Blot实验方法检测血浆Notch1、Notch2的miRNA与蛋白表达量;E2浓度与Notch1、Notch2基因和蛋白相对表达量的相关性采用Spearman秩相关进行分析。结果:血清E2检测结果显示阳虚体质组E2浓度低于非阳虚体质组(P<0.05);Notch1、Notch2的mRNA和蛋白相对表达量组间比较,差异均有统计学意义(P<0.05)。阳虚体质组Notch1、Notch2 mRNA及蛋白相对表达量均高于非阳虚体质组(P<0.001)。阳虚体质组E2水平与Notch1、Notch2 mRNA和蛋白表均存在负相关性(P<0.001)。结论:阳虚体质KOA患者中E2浓度与Notch1、Notch2基因和蛋白呈明显的负相关,推测阳虚体质患者E2水平下降,可能是促使Notch1、Notch2基因和蛋白的表达升高,各种炎性因子上升,加快KOA发生发展的潜在原因,将E2-Notch信号通路与中医体质研究相结合,寻找KOA潜在治疗靶点,可能对KOA的治疗具有一定的科学价值。Objective:To discuss the influence of the levels of estradiol(E2)in knee osteoarthritis(KOA)patients on Notch1 and Notch2 signaling pathway,and its correlational study.Methods:Sixty KOA patients were chosen,and divided into Yang deficiency constitution group and non Yang deficiency constitution group in light of the constitutions,with 30 cases in each group.After collecting peripheral venous blood on an empty stomach,electrochemical luminescence(ECL)was adopted to detect the concentrations of E2,q-PCR and Western Blotting were applied to measure the expressions of miRNA and protein of plasma Notch1 and Notch2,the correlation between the concentrations of E2,and the relative expressions of Notch1 and Notch2 gene and protein was explored using Spearman's correlation.Results:The results of serum E2 showed that the concentrations of E2 in Yang deficiency constitution group were lower than these of non Yang deficiency constitution group(P<0.05);the difference had statistical meaning in the relative expressions of mRNA and protein of Notch1 and Notch2(P<0.05).Yang deficiency constitution group was higher than non Yang deficiency constitution group in the relative expressions of mRNA and protein of Notch1 and Notch2(P<0.001).The levels of E2 were negatively related to the relative expressions of mRNA and protein of Notch1 and Notch2 in Yang deficiency constitution group(P<0.001).Conclusion:The concentrations of E2 and the expressions of Notch1 and Notch2 gene and protein represent a significant negative correlation,speculating that the decrease of E2 in Yang deficiency constitution patients might be the potential reason of raising the expressions of Notch1 and Notch2 gene and protein,the rise of the inflammatory factors and accelerating the incidence and development of KOA,the survey of the potential therapeutic targets of KOA by combining E2-Notch signaling pathway with the study on TCM constitutions might possess certain scientific value for the treatment of KOA.

关 键 词：骨关节炎 膝 NOTCH信号通路 雌二醇 中医体质 

分 类 号：R274.9[医药卫生—中西医结合]