甘草次酸对大肠癌HT-29细胞化疗增敏作用机制研究  

Chemotherapy Enhancement Effects of Glycyrrhetinic Acid on Human Colorectal Cancer HT-29 Cells

作　　者：高野 李凤岩 闫睿 白剑 苗兰英 GAO Ye;LI Fengyan;YAN Rui;BAI Jian;MIAO Lanying(The Second Hospital of Chaoyang,Chaoyang 122000,China;Laboratory Animal Center,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China)

机构地区：[1]朝阳市第二医院,辽宁朝阳122000 [2]辽宁中医药大学实验动物中心,辽宁沈阳110847

出　　处：《西部中医药》2024年第8期11-14,共4页Western Journal of Traditional Chinese Medicine

基　　金：辽宁省自然科学基金(2015020714)。

摘　　要：目的:研究甘草次酸增强奥沙利铂对大肠癌HT-29细胞化疗敏感性的作用机制。方法:将大肠癌HT-29细胞分为对照组、甘草次酸组(甘草次酸浓度为100μmol/L)、联合组(100μmol/L甘草次酸+25 mg/L奥沙利铂)和阳性对照组(奥沙利铂25 mg/L)。采用四氮唑蓝法检测各组HT-29细胞增殖率;通过Annexin V/PI双标流式细胞术检测各组HT-29细胞凋亡比例;通过蛋白质印迹法检测各组HT-29细胞中核转录因子κB(nuclear factor kappa B,NF-κB)、B淋巴细胞瘤2(B-cell lymphoma 2,Bcl-2)、激活型胱天蛋白酶3(caspase-3,CASP-3)蛋白表达。结果:与对照组相比,联合组和阳性对照组HT-29细胞增殖率降低(P<0.05),联合组和阳性对照组凋亡细胞比例均升高(P<0.05);甘草次酸组、联合组和阳性对照组NF-κB、Bcl-2蛋白相对表达量均降低(P<0.05),而激活型CASP-3蛋白表达升高(P<0.05)。结论:甘草次酸可能通过调控NF-κB信号通路增强奥沙利铂对大肠癌HT-29细胞的化疗敏感性。Objective:To investigate the mechanism of glycyrrhetinic acid enhancing the chemosentivity of oxaliplatin to human colorectal cancer HT-29 cells.Methods:Colorectal cancer HT-29 cells were allocated to the control group,glycyrrhetinic acid group(the concentrations of glycyrrhetinic acid:100μmol/L),the combination group(100μmol/L of glycyrrhetinic acid+25 mg/L oxaliplatin)and positive control group(25 mg/L oxaliplatin).Methylthiazolyl tetrazolium(MTT)was adopted to detect the proliferation rate of HT29 cells in different groups;Annexin V/PI double labeling flow cytometry was applied to measure the apoptosis ratio of HT-29 cells in different groups;Western blotting method was used to detect the expressions of NF-κB,Bcl-2 and CASP-3 in HT-29 cells in all the groups.Results:Compared with the control group,the proliferation rate of HT-29 cells was reduced in the combination group and positive control group(P<0.05),cellular apoptosis rate was raised in the combination group and positive control group(P<0.05);the relative expressions of NF-κB and Bcl-2 proteins were lowered in glycyrrhetinic acid group,the combination group and positive control group(P<0.05),while the expressions of activated CASP-3 protein were increased(P<0.05).Conclusion:Glycyrrhetinic acid could enhance the chemosentivity of oxaliplatin to human colorectal cancer HT-29 cells possibly through regulating NF-κB signaling pathway.

关 键 词：大肠癌 HT-29细胞 核转录因子ΚB 凋亡 甘草次酸 

分 类 号：R285.5[医药卫生—中药学]