牛源结核分枝杆菌pknB基因生物信息学分析  

Bioinformatics Analysis of pknB Gene of Mycobacterium tuberculosis var.bovis

作　　者：郑琛 郭明佳 Zheng Chen;Guo Mingjia(Qijia Township Animal Husbandry and Veterinary Station,Longkou 265722,Shandong,China;Longkou Center for Animal Disease Prevention and Control,Longkou 265701,Shandong,China)

机构地区：[1]龙口市七甲镇畜牧兽医站,山东龙口265722 [2]龙口市动物疫病预防控制中心,山东龙口265701

出　　处：《中国动物检疫》2024年第8期28-33,共6页China Animal Health Inspection

摘　　要：为了解牛源结核分枝杆菌关键蛋白的生物学功能,应用生物信息学方法对丝氨酸/苏氨酸蛋白激酶pknB蛋白的结构及生物学特性进行了预测。结果显示:牛源结核分枝杆菌pknB蛋白是一类Ⅱ型跨膜蛋白,其跨膜区位于蛋白中部,亲水性强但缺乏能够分泌表达的信号肽;高级结构中无规则卷曲和α螺旋占比较高,分别为44.73%和32.27%,延伸链和β转角含量较少;pknB蛋白定位在细胞内质网的可能性最高,其次为细胞质,而定位在细胞核、高尔基体和线粒体的可能性最低;存在多个优势抗原表位,其中B细胞抗原表位有12个,分值大于25分的T细胞表位有8个;存在65个潜在的磷酸化修饰位点,它们可能与MRA_3902、MRA_3400、cfp17等多种蛋白发生相互作用。结果说明:pknB蛋白作为一种跨膜蛋白具有良好的免疫原性,并参与宿主的NF-κB信号通路进而影响细菌增殖,可以作为抗结核病疫苗研发及快速诊断试剂盒研制的候选靶标蛋白。In order to verify the biological functions of key protein of Mycobacterium tuberculosis var.bovis(MTB),the structure and biological properties of serine/threonine protein kinase pknB protein were predicted using the bioinformatics method.The results showed that pknB protein belonged to type II transmembrane protein with a transmembrane region located in the middle of the protein,which was strongly hydrophilic without a signal peptide for secretion expression;random coils andα-helixes accounted for high proportions in the high-level structure,which were 44.73%and 32.27%,respectively,little extension strands andβ-turns were contained;pknB protein was most likely located in the endoplasmic reticulum of cells,then in cytoplasm,but least likely in nucleus,golgi apparatus and mitochondria;many dominant antigenic epitopes were available,including 12 B-cell antigenic epitopes and 8 T-cell epitopes with above 25 scores;there were 65 potential phosphorylation modification sites that might interact with a variety of proteins,such as MRA_3902,MRA_3400,cfp17,etc.In conclusion,as a transmembrane protein,pknB was with good immunogenicity and could affect bacterial proliferation as an actor in the NF-κB signaling pathway of hosts,hence could be used as a candidate target protein for the development of vaccines and rapid diagnostic kits of tuberculosis.

关 键 词：结核分枝杆菌 pknB基因 生物信息分析 遗传进化 

分 类 号：S851.3[农业科学—预防兽医学]