基于多指标-响应面法优化土炒白术的炮制工艺  

Process Optimization of Soil-fried Atractylodis Macrocephalae Rhizoma Based on the Multi-Index-Response Surface Method

作　　者：袁楚 刘想 高如汐 王凡一 张可[1] 吴昊 刘思奇 赵启苗 单国顺[1] YUAN Chu;LIU Xiang;GAO Ruxi;WANG Fanyi;ZHANG Ke;WU Hao;LIU Siqi;ZHAO Qimiao;SHAN Guoshun(Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Liaoning Vocational College of Medicine,Shenyang 110101,China)

机构地区：[1]辽宁中医药大学,辽宁大连116600 [2]辽宁医药职业学院,沈阳110101

出　　处：《中国现代应用药学》2024年第14期1956-1964,共9页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金青年基金项目(81803726);辽宁省教育厅面上项目(LJKMZ20222205,LJKMZ20221308);辽宁中医药大学校级课题重点项目(2021 LZY054);辽宁医药职业学院校级课题(BSKY202405)。

摘　　要：目的 对土炒白术的炮制工艺进行优化。方法 采用紫外可见分光光度法和超高效液相色谱-三重四极杆串联质谱(UPLC-QqQ-MS/MS)技术对土炒白术中多糖含量以及白术内酯Ⅰ、白术内酯Ⅱ、白术内酯Ⅲ等活性成分的含量进行测定,采用色差仪对土炒白术粉末色度值进行分析;将上述化学成分的含量以及饮片的色度作为评价指标,采用单因素试验法对土炒白术过程中的辅料用量、炒制温度、炒制时间进行考察;通过层次分析-赋值(AHP-CRITIC)混合加权法结合BoxBehnken响应面法进行土炒白术炮制工艺的优化研究。结果 优化土炒白术的最佳炮制工艺为每50 g药材用灶心土28.4 g,在204℃下炒制5.30 min。结论 本实验确定的土炒白术工艺切实可行,可为该炮制品的工业化生产提供一定的指导意义。OBJECTIVE To optimize the processing technology of soil stir-fried Atractylodis Macrocephalae Rhizoma(AMR).METHODS UV-visible spectrophotometry and ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-QqQ-MS/MS)was used to determine the content of polysaccharides and active ingredients such as atractylenolideⅠ,atractylenolideⅡ,atractylenolideⅢ,etc.The colorimeter was used to determine the color value of soil-fried AMR powder.The contents of the above chemical components and the color value of soil-fried AMR were used as evaluation indexes.The single factor trial method was adopted to investigate the amount of excipients,stir-frying temperature and time of AMR.The analytic hierarchy process and criteria importance through inter criteria correlation(AHP-CRITIC)combined with the method of Box-Behnken response surface was used to design and optimize the processing technology of soil stir-fried AMR.RESULTS The best processing technology of soil fried AMR were optimized to 28.4 g of soil added to 50 g of medicinal materials which was fried 5.30 min in 204℃.CONCLUSION The process of soil stir-fried AMR determined in this experiment is feasible which can provide certain guiding significance for the industrial production of the processed products.

关 键 词：白术 土炒 混合加权法 Box-Behnken响应面法 工艺优化 

分 类 号：R283.1[医药卫生—中药学]