黄精多糖的制备及延缓皮肤衰老活性研究  

Study on the Preparation and Delay Skin Aging Activity of Polygonatum Polysaccharide

作　　者：张文杰 付雪瑶 高艳琳 程巧鸳 ZHANG Wenjie;FU Xueyao;GAO Yanlin;CHENG Qiaoyuan(College of Pharmaceutical Science,Zhejiang Chinese Medicine University,Hangzhou 310053,China;School of Traditional Chinese Pharmay,China Pharmaceutical University,Nanjing 211198,China;Hangzhou Singclean Medical Products Co.,Ltd.,Hangzhou 310018,China;Zhejiang Institute for Food and Drug Control,Hangzhou 310052,China)

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]中国药科大学中药学院,南京211198 [3]杭州协和医疗用品有限公司,杭州310018 [4]浙江省食品药品检验研究院,杭州310052

出　　处：《中国现代应用药学》2024年第14期1972-1979,共8页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省药品监督管理局科技计划项目(2022036,2023032)。

摘　　要：目的制备多花黄精和长梗黄精2种来源黄精的多糖,优化提取工艺,对其进行理化性质分析,并评价其延缓皮肤衰老的活性。方法采用水提醇沉法,以提取温度、料液比、提取时间为考察因素,采用单因素试验及正交试验优化黄精多糖的提取条件,通过除蛋白和透析纯化多糖,采用HPLC和傅里叶红外光谱法(FT-IR)分析单糖组成及其结构,并结合体外自由基清除试验和H_(2)O_(2)诱导人皮肤成纤维细胞(HFF-1)衰老试验评价其抗氧化和延缓皮肤衰老的活性。结果黄精多糖的最佳提取工艺为提取温度80℃、料液比1∶25,提取时间1.0 h。多花黄精纯化多糖(Polygonatum cyrtonema Hua.polysaccharide,PCP)和长梗黄精纯化多糖(Polygonatum filipes Merr.polysaccharide,PFP)均含D-甘露糖、D-半乳糖醛酸、D-(+)-无水葡萄糖、D-半乳糖和DL-阿拉伯糖等5种单糖,而PFP含特征单糖鼠李糖;PCP中D-甘露糖和D-(+)-无水葡萄糖摩尔百分比较高,而PFP中D-甘露糖、D-半乳糖和D-半乳糖醛酸摩尔百分比较高,且均高于PCP。PCP中含有α和β构型的糖苷键,PFP中糖醛酸的特征显著。PCP和PFP均具有较好的自由基清除能力和延缓皮肤细胞衰老的作用,且PFP的活性更强。结论本实验通过工艺优化获得了单糖组成和含量均有所不同的PCP和PFP,两者均具有较好的延缓皮肤衰老活性,该研究结果可为黄精多糖在抗衰老化妆品中的开发应用提供科学依据。OBJECTIVE To prepare polysaccharides from two sources of Polygonatum cyrtonema Hua.and Polygonatum filipes Merr.,and to optimize the extraction process,so as to analyze the physical and chemical properties and evaluate the activity of delaying skin aging.METHODS The water extraction and alcohol precipitation method was used to optimize the extraction conditions of polysaccharides by single factor and orthogonal test with extraction temperature,solid-liquid ratio and extraction time as the investigation factors.The polysaccharides were purified by protein removal and dialysis.The monosaccharide composition and structure were analyzed by HPLC and FT-IR.The antioxidant and delay skin aging activities were evaluated by in vitro free radical scavenging test and H2O_(2)-induced human skin fibroblast(HFF-1)senescence test.RESULTS The optimum extraction conditions were as follows:extraction temperature 80℃,solid-liquid ratio 1∶25,extraction time 1.0 h.Polygonatum cyrtonema Hua.polysaccharide(PCP)and Polygonatum filipes Merr.polysaccharide(PFP)both contained D-mannose,D-galacturonic acid monohydrate,D-(+)-glucose,D-galactose and DL-arabinose,while PFP contained characteristic monosaccharide Rhamnose.The molar percentages of D-mannose and D-(+)-glucose in PCP were higher,while the molar percentages of D-mannose,D-galactose and D-galacturonic acid monohydrate in PFP were higher than those in PCP.PCP contained two kinds of glycosidic bonds ofαandβconfigurations,and the characteristics of uronic acid in PFP was significant.PCP and PFP showed good free radical scavenging ability and delay aging of skin cells,and the activity of PFP was stronger.CONCLUSION In this experiment,PCP and PFP with different monosaccharide composition and content are obtained by process optimization.Both of them have good delay skin aging activity.The results of this study could provide a scientific basis for the development and application of Polygonatum polysaccharide in anti-aging cosmetics.

关 键 词：黄精多糖 提取工艺 单糖组成 抗氧化 延缓皮肤衰老 

分 类 号：R284.1[医药卫生—中药学]