清眩润目饮对干眼模型鼠眼表炎性因子IFN-γ、IL-17、MMP3、MMP9的影响  

Effect of Qingxuan Runmu Decoction on Ocular Surface Inflammatory Factors IFN-γ,IL-17,MMP3,and MMP9 in Dry Eye Model Mice

作　　者：刘悦 王佳娣 韩其琛 赵珊珊 姚靖[1] Liu Yue;Wang Jiadi;Han Qichen;Zhao Shanshan;Yao Jing(Heilongjiang University of Chinese Medicine,Heilongjiang,Harbin 150000,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150000

出　　处：《中国中医急症》2024年第8期1336-1339,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81973908);2024年黑龙江省中医药科研项目(ZHY2024-096)。

摘　　要：目的 对雌性C57BL/6J小鼠进行干眼造模,给予中药清眩润目饮治疗,观察其对眼表炎性因子干扰素-γ(IFN-γ)、白细胞介素-17(IL-17)、基质金属蛋白酶3(MMP3)、基质金属蛋白酶9(MMP9)的影响。方法 将健康雌性C57BL/6J小鼠随机分为A组(空白对照组)、B组(模型组)、C组(中药组)、D组(玻璃酸钠组),每组20只。B、C、D组进行干眼造模,C组给予18 mg/(kg·d)的中药灌胃处理,每天2次;D组给予0.1%玻璃酸钠滴眼液,每天2次。实验结束后取小鼠的泪腺、角膜,采用免疫组化法检测IFN-γ、IL-17、MMP3、MMP9水平。结果 在小鼠泪腺组织中,与B组比较,C组IFN-γ及IL-17蛋白表达水平降低(P <0.05),MMP3蛋白表达水平显著降低(P <0.01);与B组比较,D组IFN-γ蛋白表达水平显著降低(P <0.01),MMP9蛋白表达水平降低(P <0.05)。在小鼠角膜组织中,在小鼠角膜组织中,与B组比较,C组MMP3蛋白表达水平降低(P <0.05),MMP9蛋白表达水平显著降低(P <0.01);与B组比较,D组MMP9蛋白表达水平显著降低(P <0.01)。结论 清眩润目饮能够有效下调干眼模型鼠眼表炎性因子IFN-γ、IL-17、MMP3、MMP9水平,减轻干眼眼表损伤。Objective:To investigate the effect of the traditional Chinese medicine(TCM)Qingxuan Runmu Decoction on ocular surface inflammatory factors interferon-gamma(IFN-γ),interleukin-17(IL-17),matrix metalloproteinase 3(MMP3),and matrix metalloproteinase 9(MMP9)in dry eye model mice.Methods:Healthy female C57BL/6J mice were randomly divided into four groups,each containing 20 mice:group A(blank control group),group B(model group),group C(TCM group),and group D(hyaluronic acid group).Dry eye models were created in groups B,C,and D.Group C was treated with 18 mg/kg/day of the TCM decoction via gastric gavage twice daily,and group D was treated with 0.1%hyaluronic acid eye drops twice daily.After the experiment,the lacrimal glands and corneas of the mice were collected,and immunohistochemistry was used to detect the levels of IFN-γ,IL-17,MMP3,and MMP9.Results:In the lacrimal gland tissues of the mice,compared with the B group,the expression levels of IFN-γand IL-17 proteins were decreased in the group C(P<0.05),and the expression level of MMP3 protein was significantly decreased(P<0.01).Compared with the B group,the expression level of IFN-γprotein was significantly decreased in the group D(P<0.01),and the expression level of MMP9 protein was decreased(P<0.05).In the corneal tissues of the mice,compared with the group B,the expression level of MMP3protein was decreased in the group C(P<0.05),and the expression level of MMP9 protein was significantly decreased(P<0.01).Compared with the group B,the expression level of MMP9 protein was significantly decreased in the group D(P<0.01).Conclusion:Qingxuan Runmu Decoction can effectively downregulate the levels of ocular surface inflammatory factors IFN-γ,IL-17,MMP3,and MMP9 in dry eye model mice,thereby alleviating ocular surface damage caused by dry eyes.

关 键 词：干眼 干扰素-γ(IFN-γ) 白细胞介素-17(IL-17) 基质金属蛋白酶3(MMP3) 基质金属蛋白酶9(MMP9) 清眩润目饮 小鼠 

分 类 号：R285.5[医药卫生—中药学]