蒙自藜芦中的甾体生物碱及抗炎活性研究  

Steroid alkaloids in Veratrum mengtzeanum Loes.and their anti-inflammation activity

作　　者：高文分 马金蓉 刘继华 晏润文 钱敏 卢富庆 袁文娟 GAO Wen-fen;MA Jin-rong;LIU Ji-hua;YAN Run-wen;QIAN Min;LU Fu-qing;YUAN Wen-juan(Public Service Platform for Industrial Technology Foundation of the Ministry of Industry and Information Technology,Yunnan Institute for Food and Drug Control,Kunming 650106;College of Science,Yunnan Agricultural University,Kunming 650201)

机构地区：[1]云南省食品药品监督检验研究院工业和信息化部产业技术基础公共服务平台,昆明650106 [2]云南农业大学理学院,昆明650201

出　　处：《中南药学》2024年第7期1685-1691,共7页Central South Pharmacy

基　　金：国家自然科学基金项目(No.32060084);云南省农业基础研究联合专项重点项目(No.202301BD070001-025);国家大学生创新创业训练计划支持项目(No.202210676023,No.202210676105)。

摘　　要：目的研究云南药用披麻草植物资源蒙自藜芦中的甾体生物碱成分及其抗炎活性。方法采用硅胶柱色谱法、反相柱色谱法、凝胶柱色谱法和高效液相色谱法(HPLC)制备等多种分离手段对蒙自藜芦根和根茎的乙醇提取部分进行分离,根据波谱数据确定所分离得到的甾体生物碱类化合物的结构,对这些化合物进行抗炎活性初步研究。结果从蒙自藜芦中分离出了8个甾体生物碱,并通过波谱学数据和理化性质分别鉴定为3-当归酰基棋盘花胺(1)、3-藜芦酰基计明碱(2)、介芬胺(3)、藜芦嗪(4)、vermitaline(5)、3-当归酰基棋盘花胺-β-N-氧化物(6)、环杷明(7)、藜芦胺(8)。化合物2、4、7为首次从该植物中分离得到。其中,化合物8对脂多糖(LPS)诱导的一氧化氮产生有明显的抑制作用,在50μmol·L^(-1);时一氧化氮生成抑制率为(47.01±0.41)%,且能够降低炎症因子环氧合酶(COX-2)和诱导型-氧化氮合酶(iNOS)的蛋白表达水平,并呈剂量依赖性。结论本研究可为蒙自藜芦的抗炎活性物质基础及开发利用提供科学依据。Objective To determine the steroid alkaloids and their anti-inflammation activity in Veratrum mengtzeanum Loes.,a medicinal herb in Yunnan.Methods Column chromatography,reverse phase chromatography,gel chromatography and other separation methods were used to separate and purify the ethanol extract from the roots of Veratrum mengtzeanum Loes..The chemical structures of these alkaloids were determined by spectroscopic data and physical and chemical properties,and their anti-inflammation activity was measured.Results Totally 8 steroid alkaloids,including 3-angeloylzygadenine(1),3-veratroylgemine(2),jervine(3),verazine(4),vermitaline(5),3-angeloylzygadenine-β-N-oxide(6),cyclopamine(7),and veratramine(8),were isolated and identified from the roots of Veratrum mengtzeanum Loes.Compounds 2,4,and 7 were extracted from this plant for the first time.Notably,compound 8 exhibited obvious inhibition on the production of nitric oxide induced by lipopolysaccharide.At 50μmol·L^(-1),the inhibition rate of nitric oxide production was(47.01±0.41)%.Moreover,it effectively reduced the protein expression levels of inflammation factors COX-2 and iNOS in a dose-dependent manner.Conclusion This study provides substance basis for the anti-inflammation and utilization of Veratrum mengtzeanum Loes..

关 键 词：蒙自藜芦 藜芦属 甾体生物碱 核磁共振波谱 抗炎活性 化学成分 

分 类 号：R289[医药卫生—方剂学] R285[医药卫生—中药学]