G1-熵权法结合Box-Behnken响应面法优化银星养脑方醇提工艺  

Optimization of alcohol extraction of Yinxing Yangnao formula by G1-entropy weight method combined with Box-Behnken response surface methodology

作　　者：熊丹 蒋翠平 牛江进 严建业[2] 王平杰 谢宗明[1] 高明果 陶叶琴 XIONG Dan;JIANG Cui-ping;NIU Jiang-jin;YAN Jian-ye;WANG Ping-jie;XIE Zong-ming;GAO Ming-guo;TAO Ye-qin(Liuyang Hospital of Traditional Chinese Medicine,Liuyang Hunan 410300;Hunan University of Chinese Medicine,Changsha 410006)

机构地区：[1]浏阳市中医医院,湖南浏阳410300 [2]湖南中医药大学,长沙410006

出　　处：《中南药学》2024年第7期1742-1747,共6页Central South Pharmacy

基　　金：长沙市卫健委中医药科研重点项目(No.A202304);湖南省自然科学基金项目(No.2024JJ9573);长沙市自然科学基金项目(No.kq2202497);湖南省临床医疗技术创新引导项目(No.2021SK51501)。

摘　　要：目的优化银星养脑方醇提工艺。方法基于单因素实验,以乙醇浓度、提取时间、提取溶剂倍数为影响因素,采用Box-Behnken响应面法,以阿魏酸、3,6'-二芥子酰基蔗糖、金松双黄酮、异银杏素的转移率和浸膏得率为评价指标,结合G1-熵权法计算综合评分,优选银星养脑方最佳醇提工艺。结果建立了工艺评价函数模型,模型预测最佳提取工艺为6倍量60%的乙醇提取1.4 h,提取2次。结论该提取方法稳定可行,可为银星养脑方的批量生产提供参考。Objective To optimize the alcohol extraction of Yinxing Yangnao formula.Methods Based on the single factor experiments,ethanol concentration,extraction time and times of extraction solvent were used as the influence factors.Box-Behnken response surface methodology was used,and the transfer rates of ferulic acid,3,6'-disinapoyl sucrose,sciadopitysin,isoginkgetin and extract yield as evaluation indexes.Combined with G1-entropy weight method,the comprehensive score was calculated to optimize the alcohol extraction of Yinxing Yangnao formula.Results The model predicted that the optimal extraction process was extraction with 6 times of 60%ethanol for 1.4 h and 2 extractions.Conclusion The extraction method is stable and feasible,which can provide a theoretical basis for the mass production of Yinxing Yangnao formula.

关 键 词：银星养脑方 醇提工艺:G1-熵权法 Box-Behnken响应面法 

分 类 号：R283.6[医药卫生—中药学]