DJ-1/Nrf2在精索静脉曲张相关性弱精症生精细胞氧化应激中的表达及意义  

Expression and significance of DJ-1/stable nuclear factor erythroid 2-related factor 2 in the oxidative stress of the spermatogenic cells of patients with asthenozoospermia related to varicocele

作　　者：吴元才 蔡佳豆 唐永泽 罗顺文 李晓伟 WU Yuancai;CAI Jiadou;TANG Yongze;LUO Shunwen;LI Xiaowei(Sichuan Friendship Hospital,Chengdu,Sichuan Province,610041;General Hospital form Western Theater,Chengdu,Sichuan Province)

机构地区：[1]四川友谊医院,成都610041 [2]西部战区总医院

出　　处：《中国计划生育学杂志》2024年第8期1781-1786,共6页Chinese Journal of Family Planning

摘　　要：目的:考察蛋白DJ-1(DJ-1)/稳定核转录因子红系2相关因子2(Nrf2)在精索静脉曲张相关性弱精症患者生精细胞氧化应激中的表达及意义。方法:收集本院2021年12月-2023年12月收治的120例确诊为精索静脉曲张相关性弱精症的男性患者临床资料,60例为新诊断患者纳入新诊断病例组,60例为接受维生素C联合维生素E治疗3个月的患者纳入病例治疗组;同期婚前健康检查健康男性40例为对照组。检测3组精液生精细胞形态、精液生精细胞的DJ-1、Nrf2蛋白及mRNA,外周血氧化应激[超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱苷肽过氧化物酶(GSH-Px)]、DJ-1、Nrf2。结果:3组年龄、体质指数、精液体积及精子尾部畸形率均无差异(P>0.05);对照组、病例治疗组、新诊断病例组精子正常形态依次降低,精子头畸形率、体畸形率、头体环合畸形率依次增高,生精细胞DJ-1(0.35±0.11、0.71±0.16、0.89±0.12)、Nrf2蛋白(0.31±0.09、0.62±0.14、0.78±0.13)及mRNA均依次增高,血清MDA、DJ-1、Nrf2依次增高,SOD、GSH-Px依次降低(均P<0.05)。结论:激活DJ-1/Nrf2通路有助于增强精索静脉曲张相关性弱精症生精细胞的抗氧化能力,保护细胞免受由氧化应激引起的损伤,DJ-1/Nrf2通路或是精索静脉曲张相关性弱精症的治疗的潜在分子靶点。Objective:To investigate the expression and significance of the protein DJ-1/stable nuclear factor erythroid 2-related factor 2(DJ-1/Nrf2)in oxidative stress of the spermatogenic cells of patients with asthenozoospermia related to varicocele.Methods:The clinical data of 120 male patients diagnosed with asthenozoospermia related to varicocele admitted to the hospital from December 2021 to December 2023 were collected in this study,and 60 newly diagnosed patients were included in group A,60 patients who had received treatment of vitamin C combined with vitamin E for 3months were included in group B.40 healthy males who underwent premarital health examination were collected in group C during the same time.The morphology of the semen germ cells,the levels of protein and mRNA of DJ-1 and Nrf2 of the spermatogenic cells in seminal fluid,the levels of oxidative stress indexes,such as superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH Px)in peripheral blood,and the levels of DJ-1 and Nrf2 of the males in the three groups were detected.Results:There were no significant differences in the age,the body mass index,the semen volume and the sperm tail malformation rate of the males among the three groups(P>0.05).The rate of the normal sperm morphology of the males in group C,group B and group A had decreased gradually,and the rates of the sperm head malformation,the sperm body malformation and the sperm head malformation combined the sperm body malformation of the males in group C,group B and group A had increased gradually.The DJ-1 level of the spermatogenic cells of the males in group C,group B and group A were 0.35±0.11,0.71±0.16 and 0.89±0.12,respectively,and which had increased gradually.The Nrf2 protein level of the spermatogenic cells of the males in group C,group B and group A were 0.31±0.09,0.62±0.14 and 0.78±0.13,respectively,and which had increased gradually.The levels of MDA,DJ-1 and Nrf2 of the males in group C,group B and group A had increased gradually.The levels of SOD and GSH-Px

关 键 词：弱精症 精索静脉曲张 生精细胞 蛋白DJ-1 稳定核转录因子红系2相关因子2 氧化应激 

分 类 号：R698.2[医药卫生—泌尿科学]