Eco-friendly high-throughput screening of cephalosporins impurities: Utilizing 2D-carbon microfiber fractionation system combined with quadrupole time of flight high-resolution mass spectrometer  

作　　者：Lei Yang Yan Wang Zhao Wang Meiyu Cui Ruolan Jin Hai-Bo Shang Donghao Li 

机构地区：[1]College of Pharmacy,Yanbian University,Yanji,Jilin,133002,China [2]Department of Chemistry,Interdisciplinary Program of Biological Functional Molecules,College of Integration Science,Yanbian University,Yanji,Jilin,133002,China

出　　处：《Journal of Pharmaceutical Analysis》2024年第5期772-774,共3页药物分析学报（英文版）

基　　金：supported by a grant from the National Natural Science Foundation of China(Grant Nos.:22176164 and 21904113);the Higher Education Discipline Innovation Project,China(Project No.:D18012).

摘　　要：Owing to its high sensitivity,selectivity,and accuracy,liquid chromatography coupled with mass spectrometry(LC-MS)is commonly employed to screen,confirm,and quantify impurities in drugs[1].However,LC-MS has certain drawbacks such as complicated pretreatment steps,long analysis time,large consumption of organic solvents,high maintenance costs,and,most notably,the need for high-pressure pumps and compatible hardware because of the high column backpressure[2].In this study,a two-dimensional(2D)microscale carbon fiber(CF)/active carbon fiber(ACF)system combined with a quadrupole time-of-flight high-resolution mass spectrometry(2DmCFs-QTOF-HRMS)system was developed to rapid screening impurities in the typical three generations of oral cephalosporins,i.e.,cephalexin tablets(CPXTs),cefuroxime axetil tablets(CFATs),and cefixime tablets(CFXTs)(Fig.S1).The“fuzzy chromatographic separation”sample pretreatment method separates complex samples into high-,medium-,and weak-polar fractions for successive detection of MS,achieving effective reduction of the ion suppression effect in electrospray ionization(ESI)-MS and improving the MS detection sensitivity.Compared to high performance liquid chromatography(HPLC)-MS,this method has distinct advantages such as less organic solvent consumption(1.5 mL),shorter separation and analysis times(5 min),more information on impurities,and high reproducibility.

关 键 词：system CARBON TABLETS 

分 类 号：R284.1[医药卫生—中药学] O657.63[医药卫生—中医学]