Amplifying protection against acute lung injury:Targeting both inflammasome and cGAS-STING pathway by Lonicerae Japonicae Flos-Forsythiae Fructus drug pair  

作　　者：Junjie Li Ming Dong Qing Yao Xu Dong Yuanyuan Chen Jincai Wen Yingjie Xu Zhixin Wu Xiaomei Zhao Ye Xiu Xiaoyan Zhan Zhaofang Bai Xiaohe Xiao 

机构地区：[1]Chengde Medical University,Chengde 067000,China [2]Department of Hepatology,The Fifth Medical Center of PLA General Hospital,Beijing 100039,China [3]China Military Institute of Chinese Materia,Fifth Medical Center of Chinese PLA General Hospital,Beijing 100039,China [4]National Key Laboratory of Kidney Diseases,The Fifth Medical Center of PLA General Hospital,Beijing 100039,China [5]School of Stomatology,Heilongjiang Key Lab of Oral Biomedicine Materials and Clinical Application&Experimental Center for Stomatology Engineering,Jiamusi University,Jiamusi 154007,China [6]Southern Medical University,Guangzhou 510515,China

出　　处：《Chinese Herbal Medicines》2024年第3期422-434,共13页中草药（英文版）

基　　金：granted by the Natural Science Foundation of Beijing,China(Grant No.7232321);Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine(No:ZYYCXTD-C-202005);National Natural Science Foundation of China(No.81721002).

摘　　要：Objective:Acute lung injury(ALI)is characterized by inflammation and currently lacks an efficacious pharmacological intervention.The medicine combination of Lonicerae Japonicae Flos(LJF)and Forsythiae Fructus(FF)demonstrates combined properties in its anti-infective,anti-inflammatory,and therapeutic effects,particularly in alleviating respiratory symptoms.In previous studies,Chinese medicine has shown promising efficacy in lipopolysaccharides(LPS)-induced ALI.However,there have been no reports of LJF and FF pairing for lung injury.The aim of this study is to compare the efficacy of herb pair Lonicerae Japonicae Flos-Forsythiae Fructus(LF)with LJF or FF alone in the treatment of ALI,and to explore whether LJF and FF have a combined effect in the treatment of lung injury,along with the underlying mechanism involved.Methods:A total of 36 mice were divided into six groups(control,model,LJF,FF,LF,dexamethasone)based on the treatments they received after undergoing sham-operation/LPS tracheal instillation.H&E staining and pulmonary edema indexes were used to evaluate lung injury severity.Alveolar exudate cells(AECs)were counted based on cell count in bronchoalveolar lavage fluid(BALF),and neutrophil percentage in BALF was measured using flow cytometry.Myeloperoxidase(MPO)activity in BALF was measured using enzyme-linked immunosorbent assay(ELISA),while the production of IL-1β,TNF-α,and IL-6 in the lung and secretion level of them in BALF were detected by quantitative polymerase chain reaction(qPCR)and ELISA.The effect of LJF,FF,and LF on the expression of Caspase-1 and IL-1βproteins in bone marrow derived macrophages(BMDMs)supernatant was assessed using Western blot method under various inflammasome activation conditions.In addition,the concentration of IL-1βand changes in lactatedehydrogenase(LDH)release levels in BMDMs supernatant after LJF,FF,and LF administration,respectively,were measured using ELISA.Furthermore,the effects of LJF,FF and LF on STING and IRF3 phosphorylation in BMDMs were detected by Western bl

关 键 词：acute lung injury BMDMS inflammasomes Lonicerae Japonicae Flos-Forsythia Fructus drug pair STING 

分 类 号：R285[医药卫生—中药学]