AO工艺高效处理N,N-二甲基甲酰胺废水的研究  

Study on the Efficient Treatment of N,N-dimethylforMamideWastewater by AO Process

作　　者：曹鹏飞 任学昌[1] 陈作雁 冯浩 杨镇瑜 安菊 范新露 CAO Pengfei;REN Xuechang;CHEN Zuoyan;FENG Hao;YANG Zhenyu;AN Ju;FAN Xinlu(School of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;Gansu Institute of Natural Energy,Lanzhou 730046,China)

机构地区：[1]兰州交通大学环境与市政工程学院,兰州730070 [2]甘肃省自然能源研究所,兰州730046

出　　处：《兰州交通大学学报》2024年第4期134-144,共11页Journal of Lanzhou Jiaotong University

基　　金：兰州市科技计划项目(2021-1-144)。

摘　　要：利用微生物高效降解N,N-二甲基甲酰胺(DMF)的研究是一项极具挑战的课题。通过缺氧驯化(HA)筛选并获得高效降解DMF的菌群是研究DMF废水生化降解的关键。微生物群落扩增子测序(16S rDNA)结果表明,驯化出的缺氧菌群中Proteobacteria、Firmicutes和Euryarchaeota为降解DMF的优势菌门,其中Zavarzinia、Petrimonas、Acholeplasma、Brevundimonas、Arenimonas和Bosea在缺氧条件下可以有效降解DMF,且DMF降解菌的相对丰度与DMF降解效率具有明显相关性。当HA污泥中DMF降解菌的相对丰度达到86.37%时,HA污泥对210 mg·L^(-1)的DMF去除效率达到99.98%。DMF降解菌降解DMF的产物为二甲胺(DMA)和甲胺(MMA)。DMF降解菌的相对丰度低于40.25%时,HA污泥对DMF的去除效率仅为57.22%,DMA对DMF降解菌表现出明显的抑制作用。实验采用缺氧/好氧联合(AO)工艺进一步提高了出水水质,好氧污泥(AS)出水中DMA和MMA的浓度均小于0.5 mg·L^(-1),且AS反应器出水中化学需氧量(COD)≤40.6 mg·L^(-1),该水质满足GB 21902-2008《合成革与人造革工业污染物排放标准》中对现有企业污染物排放的要求。The study of efficient degradation of N,N-dimethylformamide(DMF) using microorganisms is of great challenge.Screening and obtaining efficient DMF-degrading bacterial flora through anoxic acclimatization(HA) is the key to study DMF wastewater being biochemically degraded.The results of microbial community amplicon sequencing(16S rDNA) showed that Proteobacteria,Firmicutes,and Euryarchaeota were the dominant phyla for degrading DMF in the domesticated hypoxic bacterial community.Among them, Zavarzinia,Petrimonas,Acholeplasma,Brevundimonas,Arenimonas,and Bosea were able to effectively degrade DMF under hypoxic conditions, and the relative abundance of DMF degrading bacteria was significantly correlated with DMF degradation efficiency.When the relative abundance of DMF-degrading bacteriain HA reached 86.37%,the removal efficiency of DMF by HA sludge reached 99.98% for 210 mg·L^(-1).The products of DMF degradation by DMF-degrading bacteriawere dimethylamine(DMA) and methylamine(MMA).The removal efficiency of DMF by HA sludge was only 57.22% when the relative abundance of DMF-degrading bacteriawas lower than 40.25%,and DMA showed a significant inhibitory effect on DMF-degrading bacteria.In this study, an anoxic-oxic(AO) processwas used to further improve the effluent water quality, and the concentrations of DMA and MMA in the aerobic sludge(AS)effluent were less than 0.5 mg·L^(-1),and the chemical oxygen demand(COD) in the effluent from the AS reactor was ≤40.6 mg·L^(-1),which meets the emission standard of pollutants for synthetic leather and artificial leather industry GB 21902-2008.

关 键 词：N N-二甲基甲酰胺 生物降解 物种差异 代谢途径 

分 类 号：X703[环境科学与工程—环境工程]