猪链球菌重组磷酸ABC转运体ATP酶的免疫保护效果评价  

Evaluation of Immunoprotection of Recombinant Phosphate ABC Transporter ATPase of Streptococcus suis in Mice

作　　者：张小玲[1] 李日顺 樊擎莹 王海堃 王瑜欣 汪洋[1] ZHANG XiaoLing;LI Rishun;FAN Qingyin;WANG Haikun;WANG Yuxin;WANG Yang(Henan Provincial Engineering Research Center for Detection and Prevention and Control of Emerging Infectious Diseases in Livestock and Poultry,College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471000,China)

机构地区：[1]河南科技大学动物科技学院河南省畜禽新发传染病检测与防控工程研究中心,洛阳471000

出　　处：《中国动物传染病学报》2024年第4期53-59,共7页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金项目(321728520);河南省杰出青年科学基金项目(222300420005);河南省高校科技创新团队(24IRTSTHN033)。

摘　　要：猪链球菌(S.suis)是一种重要的人畜共患病原菌,给养猪业造成重大的经济损失。为了评估猪链球菌磷酸ABC转运体ATP酶蛋白的免疫保护效果,本研究构建了猪链球菌磷酸ABC转运体ATP酶原核表达载体进行蛋白的重组表达,纯化并制备该重组蛋白的亚单位疫苗,加强免疫接种后两周,检测其血清抗体效价、免疫保护率、各脏器载菌量以及炎性因子表达情况。结果显示,该蛋白经大肠杆菌表达后主要以可溶形式存在。ELISA检测表明该蛋白可以诱发小鼠产生高水平IgG抗体,免疫组小鼠抗体水平显著高于对照组,该重组蛋白对小鼠感染2型猪链球菌(S.suis serotype 2,S.suis 2)的保护率达到60%,显著降低了小鼠大脑、心脏及肺脏的载菌量,免疫组小鼠细胞因子IL-1β、IFN-γ、IL-6、IL-8和TNF-α的mRNA表达显著上调。研究表明猪链球菌重组磷酸ABC转运体ATP酶蛋白具有较强的免疫原性,是猪链球菌病潜在的亚单位疫苗候选蛋白。Streptococcus suis(S.suis)is one of the most important zoonotic pathogens and brings great losses to the pig industry.The objective of the present study was to evaluate the immunoprotective effect of the recombinant phosphate ABC transporter ATPase of S.suis in mice.The vector for the phosphate ABC transporter ATPase of S.suis was constructed and the recombinant ATPase protein was expressed via prokaryotic expression.The resulting recombinant ATPase protein was used to prepare a subunit vaccine for mouse immunization.The ELISA titers of serum antibodies,immunoprotection rate,bacterial loads in different organs and inflammatory factors were examined at two weeks after a booster immunization.The results showed that the recombinant protein mainly existed in supernatant of E.coli culture.The recombinant protein induced a high level of IgG antibodies and 60%immunoprotection against S.suis serotype 2 infection in mice.In addition,bacterial loads were significantly reduced in brains,hearts and lungs of the immunized mice and the mRNA expression levels of IL-1β,IFN-γ,IL-6,IL-8 and TNF-αwere also significantly up-regulated.The results from this study indicated that recombinant phosphate ABC transporter ATPase was a potential subunit vaccine candidate for prevention of S.suis serotype 2 infection.

关 键 词：猪链球菌 亚单位疫苗 磷酸ABC转运体ATP酶 免疫保护 

分 类 号：S852.4[农业科学—基础兽医学]