猪流行性腹泻病毒CH/HB/CZ01的分离鉴定及致病性研究  

Isolation,Identification and Pathogenicity of Porcine Epidemic Diarrhea Virus CH/HB/CZ01

作　　者：翟刚 刘莹[1] 赵云环 刘濮毓 王传文 李妍[1,2] 范京惠 左玉柱[1,2] ZHAI Gang;LIU Ying;ZHAO Yunhuan;LIU Puyu;WANG Chuanwen;LI Yan;FAN Jinghui;ZUO Yuzhu(College of Veterinary Medicine,Hebei Agricultural University,Baoding 071001,China;Hebei Veterinary Biotechnology Innovation Center,Baoding 071001,China)

机构地区：[1]河北农业大学动物医学院,保定071001 [2]河北省兽医生物技术创新中心,保定071001

出　　处：《中国动物传染病学报》2024年第4期97-103,共7页Chinese Journal of Animal Infectious Diseases

基　　金：河北省农业产业技术体系生猪创新团队(HBCT2018110207)。

摘　　要：为了获得猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)的地方分离株,并对其生物学特性和致病性进行初步了解。本研究在河北省开展PEDV的收集与分离鉴定,并进行遗传进化分析,同时评价分离毒株在仔猪上的致病性。将在该地区收集到的PEDV样品接种Vero细胞连续盲传,经RT-PCR检测以及基因测序鉴定,成功分离了CH/HB/CZ01毒株。利用GenBank中公布的PEDV毒株序列对该毒株进行S基因的遗传进化分析,结果显示,该毒株与我国近年来流行毒株序列的同源性为97.1%~98.3%,明显高于CV777、LZC和DR13等传统毒株的同源性,属于GⅡb亚群。将分离的PEDV病毒液按照2.0 mL/头接种健康仔猪,均出现腹泻等症状且接种2 d后直肠拭子经RT-PCR鉴定均为PEDV阳性。结果表明,分离株为变异毒株具有较强的致病性,为掌握河北省PEDV最新流行毒株的基因组特性以及生物学特性研究奠定了初步基础。In order to obtain local isolates of Porcine epidemic diarrhea virus(PEDV)for preliminary understanding of their biological characteristics and pathogenicity,virus isolation and identification were performed in in Hebei province.The isolated PEDV was analyzed for its genetic evolution and pathogenicity in piglets.The samples collected in this area were inoculated onto Vero cells for continuous blind passages and the PEDV CH/HB/CZ01 strain was isolated by RT-PCR detection and gene sequencing identification.The genetic evolution analysis of the S gene of this strain was carried out using the sequences of the PEDV references published in GenBank.The results showed that the sequence homology between this strain and the popular strains found in recent years in China was 97.1%-98.3%,which was significantly higher than that of CV777,LZC,DR13 and other traditional strains and thus this strain belonged to the GⅡb subgroup.The isolated C H/HB/CZ01 virus culture was inoculated into healthy piglets with 2.0 mL each.,The inoculated piglets developed clinical signs such as diarrhea and their rectal swabs were tested PEDV positive by RT-PCR at 2 days post inoculation.The results showed that the isolated CH/HB/CZ01 virus was a mutant with strong pathogenicity,which laid a preliminary foundation for the study of the genetic and biological characteristics of the new PEDV strains in Hebei province.

关 键 词：猪流行性腹泻病毒 遗传进化 S基因 致病性 

分 类 号：S852.65[农业科学—基础兽医学]