犬实验性感染乙脑病毒的研究  

Experimental Infection of Japanese Encephalitis Virus in Dogs

作　　者：林泠 刘巧玲 禹光美 郑如雯 李芳芳 汤德元[1] 黄涛[1] LIN Ling;LIU Qiaoling;YU Guangmei;ZHENG Ruwen;LI Fangfang;TANG Deyuan;HUANG Tao(College of Animal Science,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学动物科学学院,贵阳550025

出　　处：《中国动物传染病学报》2024年第4期118-124,共7页Chinese Journal of Animal Infectious Diseases

基　　金：青年教师国家自然科学基金培育项目建议资助项目(黔科合平台人才[2017]5788-70);贵州大学引进人才科研项目(贵大人基合字[2015]33);贵州省科技计划项目(黔科合支撑[2018]2265);贵州省2016科学技术基金(黔科合基础[2016]1048)。

摘　　要：为探讨犬感染乙型脑炎病毒(JEV)的免疫应答及临床症状。用乙型脑炎病毒强毒株贵州分离株(JEV GZ株)和乙型脑炎病毒弱毒株(SA14-14-2株)感染3月龄比格犬,感染一定时间后检测血液中免疫球蛋白M(IgM)、免疫球蛋白G(IgG)、肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)水平和T淋巴细胞亚群CD4+/CD8+T淋巴细胞变化;RT-PCR检测血液中的乙脑病毒;制作组织切片观察组织病变情况。乙型脑炎病毒强、弱毒株感染犬后,犬血清中IgM、IgG、TNF-α、IFN-γ水平升高,CD4+/CD8+比值升高;RT-PCR未检出病毒;组织切片无明显病变。犬感染乙型脑炎病毒强、弱毒株后,可产生免疫应答,无临床症状,且在组织中未检出病毒。因此,犬作为与人紧密接触的伴侣动物,非常适合作为评估人类JEV感染风险的哨兵动物。To investigate the immune response and clinical signs of Japanese encephalitis virus(JEV).3-month-old Beagle dogs were experimentally infected with a virulent JEV GZ strain from Guizhou and an attenuated SA14-14-2 strain.The serum samples were examined for changes of immunoglobulin M(IgM),immunoglobulin G(IgG),tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ)levels and T lymphocyte subsets CD4+/CD8+T lymphocytes cells.Dogs did not show clinical signs post infection with the virulent and attenuated JEV strains.The serum levels of IgM,IgG,TNF-αand IFN-γand CD4+/CD8+ratio increased.No virus was detected in blood samples by RT-PCR and also no obvious lesions were found in tissue sections.In summary,dogs infected with the virulent and attenuated JEV strains produced immune responses without clinical signs and viremia.Therefore,as companion animals live in close proximity to humans,dogs were very suitable as sentinel animals for assessing the risk of JEV infection.

关 键 词：乙脑病毒 免疫应答 哨兵动物 犬 

分 类 号：S858.292[农业科学—临床兽医学]