粤北地区鸭源沙门菌分离鉴定及生物学特性研究  

Isolation,Identification and Biological Characteristics of Salmonella from Ducks in North Guangdong Province

作　　者：南文金[1] 吴静波[1] 黄健强[1] 邬苏晓[1] 肖正中[1] 彭国良[1] 胡鸿惠[1] NAN Wenjin;WU Jingbo;HUANG Jianqiang;WU Suxiao;XIAO Zhengzhong;PENG Guoliang;HU Honghui(College of Biology and Agriculture,Shaoguan University,Shaoguan 512005,China)

机构地区：[1]韶关学院生物与农业学院,韶关512005

出　　处：《中国动物传染病学报》2024年第4期188-194,共7页Chinese Journal of Animal Infectious Diseases

基　　金：广东省科技计划项目(2021B1212050021);韶关市科技计划项目(210724154530418)。

摘　　要：为了解粤北地区鸭源沙门菌流行菌株生物学特性,本研究对鉴定的鸭源沙门菌菌株进行了血清型鉴定、毒力基因检测、药敏试验及致病性初步试验。结果显示,病死鸭组织沙门菌分离率为57.14%(16/28);鉴定为鼠伤寒沙门菌占62.5%(10/16),肠炎沙门菌占31.25%(5/16)。分离菌株最少携带了12个毒力基因,最多携带了19个毒力基因。分离菌株对阿莫西林、青霉素、罗红霉素等耐药率超过90%,对多西环素、大观霉素、环丙沙星、阿米卡星等较为敏感,所有菌株呈多重耐药。分离菌株可导致小鼠发病和部分死亡。研究结果表明,沙门菌在粤北地区发病肉鸭中有较高感染率,流行的优势血清型为鼠伤寒沙门菌和肠炎沙门菌,流行菌株普遍存在多重耐药性且具有一定的致病性。In order to understand the biological characteristics of the endemic strains of Salmonella from ducks in northern Guangdong,serotype identification,virulence gene detection and drug susceptibility test were carried out on the isolates and some strains were preliminarily tested for pathogenicity in mice.The results showed that the isolation rate of salmonella from dead ducks was 57.14%(16/28).62.5%(10/16)strains were identified as Salmonella typhimurium,31.25%(5/16)as Salmonella enteritidis.The isolated strains carried a minimum of 12 virulence genes and a maximum of 19 virulence genes.The isolates were more than 90%resistant to amoxicillin,penicillin and roxithromycin,and were more sensitive to doxycycline,macroscopycin,ciprofloxacin and amikacin,and all strains showed mult-drug resistance.The isolated strains caused morbidity and partial death in mice.The results of study showed that salmonella had a high infection rate in sick meat ducks in northern Guangdong,and the predominant serotypes were Salmonella typhimurium and Salmonella enteritidis,and the prevalent strains were generally multi-drug resistance and had a certain pathogenicity.

关 键 词：鸭 沙门菌 血清型 毒力基因 耐药性 致病性 

分 类 号：S852.61[农业科学—基础兽医学]