焦虑对雌性成年斑马鱼热耐受和游泳能力的影响  

ANXIETY ON THERMAL TOLERANCE AND SWIMMING ABILITY IN FEMALE ADULT ZEBRAFISH(DANIO RERIO)

作　　者：李小红 付成 付世建 LI Xiao-Hong;FU Cheng;FU Shi-Jian(Laboratory of Evolutionary Physiology and Behavior,Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes,Chongqing 401331,China)

机构地区：[1]重庆师范大学进化生理与行为学实验室,淡水鱼类资源保护与利用重庆市重点实验室,重庆401331

出　　处：《水生生物学报》2024年第9期1566-1572,共7页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(32370509)资助。

摘　　要：为研究焦虑对鱼类热耐受和游泳能力的影响,以雌性成年斑马鱼(Danio rerio)为研究对象,将实验鱼随机分为焦虑组和对照组,焦虑组通过2周慢性不可预测应激(Unpredictable chronic stress, UCS)处理建立焦虑斑马鱼模型,对照组不做任何处理。2周后测定两组实验鱼的焦虑行为相关指标、全身皮质醇及雌二醇水平,热耐受[临界高温(CT_(max))、临界低温(CT_(min))、致死高温(LT_(max))和致死低温(LT_(min))]及游泳能力(最大匀加速游泳速度, Ucat)。结果显示:焦虑组实验鱼的焦虑水平、皮质醇水平显著高于对照组(P<0.01),雌二醇水平显著低于对照组(P<0.01);焦虑组实验鱼的CT_(min)显著高于对照组(P<0.01), LT_(min)高于对照组(P<0.05),但两组实验鱼的CT_(max)、LT_(max)和U_(cat)差异无统计学意义(P>0.05)。研究表明:2周UCS处理能建立焦虑斑马鱼模型,焦虑引起的雌性成年斑马鱼雌激素分泌减少可能对斑马鱼后期的繁殖产生消极影响;另外,焦虑降低了斑马鱼的低温耐受能力,可能与皮质醇水平升高和焦虑导致的体质下降有关。To investigate the impact of anxiety on the thermal tolerance and swimming ability of fish,this study focuses on female adult zebrafish(Danio rerio),where experimental subjects were randomly divided into anxious and control groups.The anxious group underwent a 2-week treatment of chronic unpredictable stress(UCS)to establish an anxious model,while the control group remained untreated.Following the treatment period,anxiety-related behavioral indica-tors,whole-body cortisol and estradiol levels,thermal tolerance[Critical thermal maximum(CT_(max)),critical thermal minimum(CT_(min)),lethal thermal maximum(LT_(max)),and lethal thermal minimum(LT_(min))],and swimming ability(Maximum sustained swimming speed,U_(cat))were assessed in both groups.The results showed significantly heightened anxiety levels and cortisol concentrations in the anxious group compared to the control group(P<0.01),accompanied by notably lower estradiol levels(P<0.01).The CT_(min) of the anxiety group was significantly elevated in contrast to the control group(P<0.01),and LT_(min) also showed a higher trend(P<0.05),although no statistically significant diffe-rences were observed in CT_(max),LT_(max),and U_(cat) between the two groups(P>0.05).The study suggests that a 2-week UCS regimen effectively establishes an anxiety model in zebrafish,and the consequential reduction in estradiol secre-tion in adult female may detrimentally impact reproduction in the later stages.Additionally,anxiety attenuates the cold tolerance of zebrafish,which may be related to the elevation of cortisol levels and the decrease in physical fitness caused by anxiety.

关 键 词：焦虑 热耐受 最大匀加速游泳速度 慢性不可预测应激 斑马鱼 

分 类 号：Q178.1[生物学—水生生物学]