马里苷减轻高糖诱导的大鼠心肌细胞系H9c2损伤  

Marein alleviates high glucose-induced damage of rat myocardial cell line H9c2

作　　者：祖力皮亚·阿布拉 赵强[2] 张泰民 李甜[4,5] Zulipiya·ABULA;ZHAO Qiang;ZHANG Taimin;LI Tian(School of Traditional Chinese Medical,Xinjiang Medical University,Urumqi 830001;Heart and Pan-vascular Medical Diagnosis and Treatment Center,People′s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830001,China;Clinical Medicine Department,Xinjiang Medical University,Urumqi 830001;Department of Histology and Embryology,School of Basic Medicine,Xinjiang Medical University,Urumqi 830001;Xinjiang Key Laboratory of Molecular Biology of Endemic Diseases,Xinjiang Medical University,Urumqi 830001)

机构地区：[1]新疆医科大学中医学院,新疆乌鲁木齐830001 [2]新疆维吾尔自治区人民医院心脏及泛血管医学诊疗中心,新疆乌鲁木齐830001 [3]新疆医科大学临床医学部,新疆乌鲁木齐830001 [4]新疆医科大学基础医学院组织学胚胎学教研室,新疆乌鲁木齐830001 [5]新疆医科大学新疆地方病分子生物学重点实验室,新疆乌鲁木齐830001

出　　处：《基础医学与临床》2024年第9期1269-1273,共5页Basic and Clinical Medicine

基　　金：新疆维吾尔自治区高校科研计划(XJEDU2020Y021);国家级大学生创新创业训练计划(202010760011);新疆医科大学博士启动基金(2023)。

摘　　要：目的研究马里苷对高糖所致大鼠心肌细胞系(H9c2)损伤的影响。方法将H9c2细胞分为对照组(Ctrl)、高糖(30 mmol/L)损伤模型组和马里苷(25、50、100μmol/L)干预模型组。MTT法检测H9c2细胞的活性;Western blot检测细胞LC-3Ⅱ/Ⅰ、p62、mTOR蛋白表达。结果与对照组相比,模型组细胞活性下降(P<0.01),马里苷组H9c2细胞活性显著增强(P<0.01);模型组LC-3Ⅱ/Ⅰ蛋白表达水平降低(P<0.05),p62及mTOR水平升高(P<0.01),马里苷组LC-3Ⅱ/Ⅰ水平增加,p62及mTOR降低(P<0.01),且随着浓度的增加蛋白质表达量更加明显。结论马里苷能够影响高糖诱导的心肌细胞损伤的自噬相关蛋白质的表达。Objective To study the effect of marein on the damage of rat myocardial cell line(H9c2)induced by high glucose.Methods H9c2 cells were divided into Ctrl group,high glucose(30 mmol/L)injury model group and marein(25,50,100μmol/L)intervention group.The viability of H9c2 cells was detected by MTT method.Western blot was used to detect the protein expression level of LC-3Ⅱ/Ⅰ,p62 and mTOR.Results Compared with the Ctrl group,the viability of H9c2 cells in the model group was decreased(P<0.01)and the viability of H9c2 cells in the marein group was significantly increased(P<0.01).The protein expression of LC-3Ⅱ/Ⅰin model group was decreased(P<0.05)and the level of p62 and mTOR was increased(P<0.01).The level of LC-3Ⅱ/Ⅰin the marein group was increased,while the level of p62 and mTOR was decreased(P<0.01).The protein expression was more obvious with the increase of concentration.Conclusions Marein can affect the expression of autophagy associated proteins in cardiomyocyte injury induced by high glucose.

关 键 词：马里苷 H9C2细胞 高糖损伤 自噬 

分 类 号：R285.5[医药卫生—中药学]