细胞连接和细胞外基质相关基因在扩张皮肤新生中的表达分析  

Expression analysis of genes related to cell junction and extracellular matrix in expanded skin regeneration

作　　者：刘维 余州 马显杰 LIU Wei;YU Zhou;MA Xianjie(Department of Plastic Surgery,Xijing Hospital,Air Force Medical University,Xi'an 710032,China)

机构地区：[1]空军军医大学西京医院整形外科,陕西西安710032

出　　处：《空军军医大学学报》2024年第8期852-858,共7页Journal of Air Force Medical University

基　　金：国家自然科学基金面上项目(81971851,82172229)。

摘　　要：目的利用生物信息学方法分析细胞连接和细胞外基质(ECM)相关基因在扩张皮肤中的表达。方法下载GEO数据库中大鼠头部扩张皮肤和小鼠背部扩张皮肤的高通量测序数据,筛选差异表达基因(DEGs),对两组DEGs集富集的生物学过程进行GO富集分析和KEGG富集分析,观察扩张皮肤新生过程中细胞连接和ECM相关基因的表达,使用CytoHubba软件分析扩张皮肤新生中的Hub基因。结果大鼠头部扩张皮肤和小鼠背部扩张皮肤的基因表达集中相同的基因共有287个。GO分析和KEGG分析显示两个DEGs集和共有DEGs均富集在表皮发育、细胞细胞连接组织、细胞细胞黏附、ECM的组织、IL-17信号通路上,其中细胞细胞连接基因共有23个,包括黏附连接(Cdh2、Cdh3)和缝隙连接(Gjb2、Gjb6)等在扩张皮肤中表达上调,ECM基因共有15个,包括Serpinb5、Klk7、Mpzl3、Tgm1、Fermt1在扩张皮肤中表达上调。此外,我们表征了富集在表皮发育、细胞细胞连接组织、ECM的组织、细胞细胞黏附的共有DEGs。结论通过生物信息学方法,我们进一步了解细胞连接和ECM相关基因在扩张皮肤新生过程中的表达,为研究机械牵拉介导的扩张皮肤新生机制提供了新的思路。Objective To analyze the expression of genes related to cell junction and extracellular matrix(ECM)in expanded skin by bioinformatic methods.Methods The high-throughput sequencing data of the expanded skin on rats scalp and the expanded skin on the back of mice were downloaded from GEO database,the differentially expressed genes(DEGs)were screened,the biological processes of DEGs sets in the two groups were analyzed by GO enrichment analysis and KEGG enrichment analysis,and the expression of genes related to cell junction and ECM were observed during expanded skin regeneration.In addition,CytoHubba was used to analyze Hub genes in expanded skin regeneration.Results There were 287 identical genes in the expanded scalp of the rat and the expanded skin of the mice back.GO analysis and KEGG analysis showed that the two DEGs sets and common DEGs were enriched in epidermal development,cell-cell junction tissue,cell-cell adhesion,ECM tissue,and IL-17 signaling pathway,among which 23 cell-cell junction genes were up-regulated in expanded skin,including adhesion junctions(Cdh2,Cdh3)and gap junctions(Gjb2,Gjb6),and 15 ECM genes were up-regulated in expanded skin,including Serpinb5,Klk7,Mpzl3,Tgm1,and Fermt1.Moreover,the common DEGs enriched in epidermal development,cell-cell junction tissue,ECM tissue,and cell-cell adhesion were characterized.Conclusion Through bioinformatic methods,we further understand the expression of genes related to cell junction and ECM in the process of expanded skin regeneration,providing a new way to study the mechanism of mechanical stretch-mediated expanded skin regeneration.

关 键 词：皮肤软组织扩张术 细胞连接 细胞外基质 转录组学 

分 类 号：R622[医药卫生—整形外科]