响应面法优化低共熔溶剂提取吴茱萸生物碱  

Optimization of alkaloids extraction from Euodia rutaecarpa with deep eutectic solvents by response surface methodology

作　　者：王楠 密成杰 张春楠 黄姝[2] 赵楠 WANG Nan;MI Chengjie;ZHANG Chunnan;HUANG Shu;ZHAO Nan(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang Province,China;College of Chemistry and Chemical,Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang Province,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011 [2]牡丹江师范学院化学化工学院,黑龙江牡丹江157011

出　　处：《化学工程》2024年第8期12-17,共6页Chemical Engineering(China)

基　　金：黑龙江省省属高校基本科研业务费(1452MSYQN008);牡丹江师范学院科研项目(MNUQN202304);牡丹江师范学院研究生创新项目(kjcx2022-051mdjnu)。

摘　　要：通过响应面法优化超声法辅助低共熔溶剂提取吴茱萸中生物碱的提取工艺。制备多种低共熔溶剂,通过供氢体、受氢体以及醇类碳链长度和羟基数量的筛选,采用最佳低共熔溶剂体系对吴茱萸生物碱进行提取,探讨低共熔溶剂的摩尔比、含水量(体积分数)以及提取时间、提取温度和料液比对生物碱提取率的影响。结果表明:以乙酰丙酸和1,4-丁二醇为受氢体和供氢体制备低共熔溶剂,在摩尔比2:1、含水量30%、提取时间50 min、提取温度30℃、料液比1:40 g/mL超声提取条件下,吴茱萸生物碱提取率达2.52%,是传统有机溶剂乙醇提取的3.2倍,低共熔溶剂在提取生物碱成分方面具有一定发展潜力。Response surface method was used to optimize the alkaloids extraction from Euodia rutaecarpa with ultrasonic-assisted deep eutectic solvent.By screening hydrogen donors,hydrogen recipients,carbon chain length of alcohols and number of hydroxyl groups,a variety of deep eutectic solvents were prepared to extract alkaloids from Euodia rutaecarpa.The extraction rate of alkaloids were discussed through molar ratio,water content(volume fraction),extraction time,extraction temperature and solid-liquid ratio.The results show that under the conditions of molar ratio 2:1,water content 30%,extraction time 50 min,extraction temperature 30℃and solid-liquid ratio 1:40 g/mL,the extraction rate of alkaloids reaches 2.52%,which is 3.2 times of ethanol extraction.Deep eutectic solvents have certain potential in extracting alkaloids.

关 键 词：吴茱萸 生物碱 低共熔溶剂 响应面法 提取工艺 

分 类 号：R931.6[医药卫生—生药学]