不同中间砧对富士苹果贮藏期表皮蜡质和角质的影响  

Effects of different intermediate rootstocks on epidermal wax and cuticle of Fuji apple fruits during storage

作　　者：李芳红 杨瑞瑞 白露 王雪雪 李欣 王延秀[2] 毕阳[1] LI Fanghong;YANG Ruirui;BAI Lu;WANG Xuexue;LI Xin;WANG Yanxiu;BI Yang(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,Gansu,China;College of Horticulture,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学食品科学与工程学院,兰州730070 [2]甘肃农业大学园艺学院,兰州730070

出　　处：《果树学报》2024年第8期1604-1616,共13页Journal of Fruit Science

基　　金：(701)校科研项目统管(0722089)。

摘　　要：【目的】对果实表皮的蜡质和角质层组分进行定性和定量测定,探究果实表皮蜡质和角质对果实采后贮藏的影响,以提高果实贮藏品质。【方法】比较SH6和M26作中间砧的富士苹果在常温贮藏期间的失重率和呼吸强度,通过扫描电子显微镜观察表皮蜡质形态和结构的差异,并采用气相色谱/质谱联用仪测定蜡质和角质的单体含量。【结果】常温贮藏期间,SH6果实的失重率和呼吸强度显著低于M26果实,表皮蜡质层厚度、蜡质和角质总量显著高于M26果实。两种砧木果实的果皮中鉴定出28种角质共有成分,34种蜡质共有成分,其中角质共有成分包括14种脂肪酸、7种羟基脂肪酸、2种二羧酸和5种醇。此外,SH6果实还有5种特有角质成分,包括花生四烯酸、顺式-8,11,14-二十烷三烯酸、18-羟基-9,12-十八碳二烯酸、22-羟基二十二烷酸和二十四烷醇。M26果实也有4种特有角质成分,包括十八烷酸、(3β)-3-三羟基-齐墩果-12-烯-28-酸、10,13-二十碳二烯酸和7,10,13-二十碳三烯酸。两种砧木果实的果皮中蜡质共有成分包括5种烷烃、6种醇、4种脂肪酸、12种酯类、2种醛类和4种其他物质。SH6果实还有3种特有蜡质成分,包括熊果醛、二十烷酸丁酯和熊果酸甲酯。M26果实也有3种特有蜡质成分,包括二十烷酸、棕榈酸-3-甲基丁酯和山嵛酸乙酯。相关性分析表明,果实贮藏期间的失重率和呼吸强度与蜡质层厚度呈显著负相关(r=-0.85,r=-0.77),失重率与羟基脂肪酸和酯类含量呈显著正相关(r=0.81,r=0.87),呼吸强度与羟基脂肪酸和二羧酸含量呈正相关(r=0.59,r=0.50)。【结论】与M26作中间砧相比,SH6作中间砧的富士苹果果实具有较厚的表皮蜡质层和更丰富的角质和蜡质含量,使其在贮藏期间具有更低的失重率和呼吸速率。【Objective】Apple skin wrinkling,caused by water loss during storage,is a critical factor that impacts the quality and taste of the fruit.The cuticle and wax layer of apple fruits play an important role in inhibiting water transpiration and respiration.Therefore,the qualitative and quantitative determination of wax and cuticle fractions in apple fruits during storage can help enhance fruit storage practices in the future.Although studies have been conducted on the effects of various rootstocks on apple fruit quality,antioxidant activity and disease resistance,the impact of different rootstocks on fruit wax and cuticle during apple storage has not been reported.SH6 and M26 are two types of dwarf interstocks commonly utilized in the cultivation of Fuji apples in Northwest China.The former was selected by Shanxi Pomology Institute,and the latter was chosen by East Malling Experimental Station in Britain.In this study,Fuji apple trees on SH6 and M26 intermediate rootstocks were used as test materials.Comparison of differences in weight loss,respiration intensity,and epidermal wax morphology and structure during storage of Fuji apples with SH6 and M26 as intermediate rootstocks was carried out.【Methods】In this study,the weight loss of fruits growing on two types of rootstocks during storage was measured using the gravimetric method.The changes in respiration rate of fruits growing on two types of rootstocks during ambient storage were measured using a fruit and vegetable measuring instrument.Morphology of the epidermal wax layer was observed under a scanning electron microscopy.The waxy layer and cuticle of the fruit epidermis were extracted using organic solvents like chloroform and methanol.The total amount of cutin and wax,as well as the content of various monomer components,were analyzed using GC-MS.In order to compare the storage characteristics of fruits,the differences in horny and wax morphology and composition between the two rootstocks were examined.【Results】The results showed that the weight loss

关 键 词：苹果果实 中间砧 贮藏性 角质 蜡质 

分 类 号：S661.1[农业科学—果树学]