黄烷酮-7-O-葡萄糖基转移酶(UF7GT)的生物信息学分析  

Bioinformatics Analysis of UDP-glucose:flavanone 7-O-glucosyltransferase(UF7GT)

作　　者：肖妃垚 刘娟[1,2] 黄旭[1,2] 田苗苗[1,2] 单杨 Xiao Feiyao;Liu Juan;Huang Xu;Tian Miaomiao;Shan Yang(Longping Branch Graduate School,Hunan University,Changsha,410082;Institute of Agricultural Product Processing,Hunan Academy of Agricultural Sciences.Hunan Province Key Lab of Fruits,Vegetables Storage,Processing,Quality and Safety/Hunan Province International Joint Lab on Fruits,Vegetables Processing,Quality and Safety,Changsha,410125)

机构地区：[1]湖南大学隆平分院,长沙410082 [2]湖南省农业科学院,农产品加工研究所,果蔬贮藏加工与质量安全湖南省重点实验室,湖南省果蔬加工与质量安全国际科技创新合作基地,长沙410125

出　　处：《分子植物育种》2024年第16期5221-5234,共14页Molecular Plant Breeding

基　　金：湖南省科技厅重点领域研发技术项目(2019NK2041);湖南省农业科技创新资金重点项目(2020CX47);国家科技支撑计划项目(2012BAD31B02)共同资助。

摘　　要：黄烷酮-7-O-葡萄糖基转移酶(UDP-glucose:flavanone 7-O-glucosyltransferase,UF7GT)是柑橘类植物中黄烷酮-7-O-葡萄糖苷类化合物生物合成途径的关键酶之一。本研究利用生物信息学相关分析工具对不同植物来源的43个UF7GT的一级结构、二级结构及三级结构等进行分析与预测,并构建了该家族系统进化树。结果表明,UF7GT基因开放阅读框架(open reading frame,ORF)为1326~1926 bp,编码440~500个氨基酸,理论等电点均低于7,大部分是亲水蛋白,不含信号肽和导肽,部分稳定;除葛麻姆来源的UF7GT蛋白含两个跨膜螺旋结构,其他均无跨膜结构;亚细胞定位预测结果表明,UF7GT最大可能定位在质膜或细胞质上;二级结构组成比例相似,α-螺旋和无规则卷曲是主要的结构元件,属于GT-B超家族成员,多数蛋白在进化上高度保守。分析结果为植物UF7GT的功能研究及黄烷酮糖苷的生物合成提供理论依据。UDP-Glucose:flavanone 7-O-glucosyltransferase(UF7GT)catalyzes the final step of flavanone biosynthesis to form flavanone-7-O-glucoside in the circus.In this paper,the nucleic acid sequences and amino acid sequences of UF7GT from 43 species registered in GenBank were analyzed by bioinformatics methods,including the primary structure,secondary structure,tertiary structure,and phylogenetic tree,etc.The results showed that the open reading frames(ORF)of UF7GT genes were about 1326~1926 bp,encoded 440~500 amino acids,and the isoelectric point was all lower than 7.Further,the investigation found that UF7GT in these species without leader peptides and signal peptides in N-terminal,the polypeptide chain presented hydrophilic and most of these UF7GT proteins are structural stable.The UF7GT proteins have non-transmembrane structures but two transmembrane helical structures in Pueraria montana var.Lobata.The subcellular localization analysis found that existing and working on the plasma membrane or cytoplasm.The main motifs of the predicted secondary structure of UF7GT are alpha-helix and random coil,which belong to the GT-B superfamily,and most of them are highly conserved in evolution.The research would provide the basis for further investigating the function and structure characteristics of UF7GT and the biosynthesis of flavanone glycosides in the future.

关 键 词：黄烷酮-7-O-葡萄糖苷类化合物 黄烷酮-7-O-葡萄糖基转移酶 生物信息学 

分 类 号：Q811.4[生物学—生物工程] S66[农业科学—果树学]