利用F_(2:3)群体对甜玉米籽粒性状的QTL定位  

QTL Mapping for Kernel Related Traits Using Sweet Corn F_(2:3) Populations

作　　者：党冬冬 秦涛 于典司[1,2,3] 王慧[1,2,3] 潘广磊 阮燕晔[4] 宋忠明 关媛[1,2,3] 郑洪建[1,2,3] Dang Dongdong;Qin Tao;Yu Diansi;Wang Hui;Pan Guanglei;Ruan Yanye;Song Zhongming;Guan Yuan;Zheng Hongjian(Crop Breeding&Cultivation Research Institution,CIMMYT-China Specialty Maize Research Center,Shanghai Academy of Agricultural Sciences,Shanghai,201403;Shanghai Engineering Research Center of Specialty Maize,Shanghai,201403;Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai,201403;College of Biological Science and Technology,Shenyang Agricultural University,Shenyang 110866;Shanghai Municipal Agricultural Technology Extension and Service Center,Shanghai,201103)

机构地区：[1]上海市农业科学院作物育种栽培研究所CIMMYT-中国特用玉米研究中心,上海201403 [2]上海市农业科学院,上海特用玉米工程技术研究中心,上海201403 [3]上海市农业科学院,上海市农业遗传育种重点实验室,上海201403 [4]沈阳农业大学生物科学技术学院,沈阳110866 [5]上海市农业技术推广服务中心,上海201103

出　　处：《分子植物育种》2024年第16期5317-5325,共9页Molecular Plant Breeding

基　　金：上海市科技兴农项目(沪农科创字(2019)第1-1号);上海市科技计划项目(20392000400)共同资助。

摘　　要：籽粒大小和重量是玉米产量的重要组成部分,同时籽粒大小也是影响外观品质的重要因素。本研究利用甜玉米自交系SHL03和SHL01为亲本构建的F_(2:3)群体,对完熟期玉米籽粒性状的粒长、粒宽、籽粒直径和百粒重进行QTL定位。共定位到35个相关的QTL,位于玉米的10条染色体上,其中百粒重QTL 5个,籽粒大小QTL 30个,解释表型变异的2.44%~12.2%。主效QTL qKD10、qHKW7、qKW6的表型贡献率分别为11.44%、12.2%、10.15%。在3、6、9、10号染色体上同一区域内检测到了控制不同性状的基因组区段。从主效QTL的置信区间共找到27个注释基因,根据功能注释以及表达谱分析筛选出候选基因Zm00001d023994、Zm00001d035561、Zm00001d024027、Zm00001d020325、Zm00001d020374、Zm00001d020311、Zm00001d020409直接或者间接影响着玉米籽粒的发育。研究结果为甜玉米籽粒大小和百粒重性状的遗传研究和分子标记辅助育种提供了一定帮助。The kernel size and weight are important components of grain yield,and kernel size is also an important factor affecting appearance quality in maize.In this study,QTL of kernel length,kernel width,kernel diameter and 100-kernel weight were mapped using F_(2:3) population derived from sweet corn inbred lines SHL03 and SHL01 as parents.Five QTL for 100-kernel weight and thirty QTL for kernel size were detected,and interpreting 2.44%~12.2%of the phenotypic variation.Three major QTL,qKD10,qHKW7 and qKW6,were interpreting 11.44%,12.2%,and 10.15%of phenotypic variation,respectively.Genome segments controlling different traits were detected in the same regions on 3,6,9,and 10 chromosomes.According to expression profile analysis and functional annotation,a total of 27 candidate genes were found on the confidence intervals of major QTL.It was predicted that maize genes Zm00001d023994,Zm00001d035561,Zm00001d024027,Zm00001d020325,Zm00001d020374,Zm00001d020311,and Zm00001d020409 would be involved in formation of kernel size.These results would helpful for genetic study on kernel size and 100-kernel weight traits,and provide marker for molecular-assisted selection of kernel size traits in breeding of sweet corn.

关 键 词：甜玉米 籽粒大小 百粒重 QTL 

分 类 号：S513[农业科学—作物学]